Donath-Landsteiner test

Donath-Landsteiner test

 [do´nath land´sti-ner]
a test for paroxysmal hemoglobinuria based on the fact that the blood of patients with this disease contains isohemolysin and autohemolysin that unites with erythrocytes only at low temperatures (2° to 10°C), hemolysis occurring only after warming with the complement to 37°C.
References in periodicals archive ?
A Donath-Landsteiner test can be used to detect the presence of the implicated biphasic hemolysin.
Direct Donath-Landsteiner Test. We collected two samples of venous blood into tubes without anticoagulant, previously warmed at 37[degrees]C.
Although the test was positive, we proceeded to confirm the presence of antibody by indirect Donath-Landsteiner test.
(1) In most cases plasma is sufficient, except, for example, in some adsorption studies and would be necessary in testing that required complement such as the Donath-Landsteiner test.
The unique biphasic nature of this condition can be demonstrated in vitro using the Donath-Landsteiner test. This test allows for the macroscopic visualization of hemolysis in specimens that are cooled and then heated to 37[degrees]C, as detailed in the AABB technical manual.