A Donath-Landsteiner test
can be used to detect the presence of the implicated biphasic hemolysin.
Direct Donath-Landsteiner Test. We collected two samples of venous blood into tubes without anticoagulant, previously warmed at 37[degrees]C.
Although the test was positive, we proceeded to confirm the presence of antibody by indirect Donath-Landsteiner test.
(1) In most cases plasma is sufficient, except, for example, in some adsorption studies and would be necessary in testing that required complement such as the Donath-Landsteiner test
The unique biphasic nature of this condition can be demonstrated in vitro using the Donath-Landsteiner test
. This test allows for the macroscopic visualization of hemolysis in specimens that are cooled and then heated to 37[degrees]C, as detailed in the AABB technical manual.