We detected significant decreases in total thiol level, native thiol level, and native thiol/total thiol ratio, but significant increases in disulphide level, disulphide/native thiol ratio, and disulphide/total thiol ratio in the AA patients.
(8) reported that disulphide/thiol ratio increased in chronic urticaria and increased oxidative stress reduced disulphide ratio in chronic stage.
Histochemical examination of hair has shown that in the trichilemmal sac encircling hair the germinal cells rich in free thiol groups are abundant, and disulphide bonds limit themselves (24).
In our study, disulphide levels and the disulphide/native thiol and disulphide/total thiol ratios that reflect the OS were found to be higher in the PCOS group than in the non-PCOS group, and native thiol levels and the native thiol/total thiol ratio, the markers of the antioxidant system, were observed to be decreased in the PCOS group.
In conclusion, thiol/disulphide homeostasis is deteriorated, and disulphide levels are increased in PCOS.
Follicular fluid thiol/disulphide homeostasis between PCOS and non-PCOS group PCOS group n=22 Non-PCOS group n=20 Native thiol ([micro]mol/L) 297.1[+ or -]37.3 334.2[+ or -]72.5 Total thiol ([micro]mol/L) 323.8[+ or -]37.3 352[+ or -]72.4 Disulphide ([micro]mol/L) 13.2[+ or -]2.7 8.5[+ or -]3.2 Disulphide/native thiol (%) 4.5[+ or -]1.2 2.8[+ or -]1.2 Disulphide/total thiol (%) 4.1[+ or -]1 2.5[+ or -]1.2 Native thiol/total thiol (%) 91.8[+ or -]1.9 95[+ or -]2.3 p value Native thiol ([micro]mol/L) 0.041(t) Total thiol ([micro]mol/L) 0.11 (t) Disulphide ([micro]mol/L) <0.0001 (t) Disulphide/native thiol (%) <0.0001 (t) Disulphide/total thiol (%) <0.0001 (t) Native thiol/total thiol (%) <0.0001 (t) (t): Student's t-test; PCOS: polycystic ovary syndrome
A) Chemical structures of reactive disulphides and the redox potentials ([[E.sub.1-2]) determined by rotating disk-electrode voltammetry.
(24, 47, 48) Mutation of Cys616 and Cys621 in rat TRPV1, which are located in the pore-forming domain between the fifth and sixth transmembrane regions, caused significant suppression of the TRPV1 activation by reactive disulphide, NO and [H.sub.2][O.sub.2].
Total thiol, native thiol, and disulphide levels were measured by a new automated method.
However, WBC, serum levels of raftlin, presepsin, disulphide and CRP, and the ratios of disulphide to total thiol and disulphide to native thiol were higher in patients compared to the controls(p0.05) difference between the non-perforated and perforated appendicitis patients with regard to all the measured parameters except mean Alvarado scores (Table2).
Keywords: Disulphide, thiol, oxidative stress, silica, exposure
Thiols may go through oxidation by oxidants thus covalent disulphide bonds are formed.