direct antiglobulin test


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Related to direct antiglobulin test: Coombs test, Indirect Antiglobulin Test

direct antiglobulin test

Direct Coombs test Immunology A test to detect immune hemolysis caused by binding of Ig and/or complement components to RBCs after sensitization to an antigen–eg Rh factor–on the RBC surface; a DAT helps differentiate autoimmune and 2º immune hemolytic anemia, which can be drug-induced or associated with underlying disease such as lymphoma. See Indirect antiglobulin test.

direct antiglobulin test

Abbreviation: DAT
A laboratory test for the presence of complement or an antibody that is bound to a patient's red blood cells (RBCs). The test is used in patients with autoimmune hemolytic anemia, hemolytic disease of the newborn, and transfusion reactions. After the patient's RBCs are washed to remove unbound antibodies, they are mixed with antihuman globulin serum containing polyvalent antibodies that bind with the antibody or complement on the RBCs and cause them to agglutinate (clump). Monoclonal antibodies can be used to identify the specific class of antibody or complement component causing RBC destruction. See: Coombs' test
References in periodicals archive ?
How to use: the direct antiglobulin test in newborns.
* A positive direct antiglobulin test (DAT) without clinically evidence of hemolysis may be found in 7-14% of patients [5].
Bel and Ribera had opined that elution was positive in all direct antiglobulin test positive ABO incompatible newborn.
F.: Incidence of antibody formation and positive direct antiglobulin tests in a multi-transfused hemodialysis population: Transfusion 24:198-200, 1984.
Concurrent AIHA cannot be excluded, especially in view of the positive IgG result in the direct antiglobulin test, and may have contributed to the severity of the patient's hemolytic anemia.
The standard Ortho Clinical Diagnostic transfusion log is a commercially designed, large multicolumn and multi-row log that includes major columns with subdivisions for the date, patient identification, donor identification, blood type and screen, antibody identification, direct antiglobulin test, compatibility testing, component issuance and disposal and with a column for the technologist involved in testing, as well as a column for additional information.
Already approved for patient and donor ABO/Rh testing, antibody screening using a pooled, two-or three-cell screen, and donor ABO and RH confirmation testing, the expanded test menu options include antibody ID-8 and ID-11 cell panels; weak D testing; Rh and Kell phenotyping; cross match; and direct antiglobulin test. This next-generation analyzer offers seven-day, on-board storage of liquid and microplate reagents, and has advanced intuitive features, including automated quality-control testing, batch validation, on-demand volume verification, automated, streamlined maintenance, and new reporting capabilities.

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