direct immunofluorescence


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direct immunofluorescence (DIF),

fluorescence microscopy of tissue from lesions after application of labeled antibodies.
See also: fluorescent antibody technique.

direct im·mu·no·fluor·es·cence

(dĭr-ekt' im'yū-nō-flōr-es'ĕns)
Fluorescence microscopy of tissue from lesions after application of labeled antibody.
See also: fluorescent antibody technique
References in periodicals archive ?
In all patients, direct immunofluorescence assay revealed IgG/C3 linear deposits along the BMZ.
(6.) Raghu AR, Nirmala NR, Sreekumaran N: Direct immunofluorescence in oral lichen planus and oral lichenoid reactions.
Comparison of a commercial qualitative real-time RT-PCR kit with direct immunofluorescence assay (DFA) and cell culture for detection of influenza A and B in children.
(13.) Thomas EE, Book LE: Comparison of two rapid methods for detection of respiratory syncytial virus (RSV) (TestPack RSV and Ortho RSV ELISA) with direct immunofluorescence and virus isolation for the diagnosis of pediatric RSV infection, J Clin Microbiol 1991; 29: 632-5.
Direct immunofluorescence consists of the identification of elementary corpuscles in endocervical material, through fluorescent monoclonal antibodies, identified with an optical microscope [9,10].
(3) The sine qua non of the disease, though, is demonstration through direct immunofluorescence of complement deposition and IgG in a linear band along the basement membrane.
Fecal positive samples were examined for Cryptosporidium and Giardia cysts by direct immunofluorescence staining technique, using a commercial kit (Meriflour-Meridian Diagnostics, Inc., Cincinnati, Ohio).
Skin biopsies were performed, including for direct immunofluorescence.
Keywords: Direct immunofluorescence, Tzanck smear, pemphigus vulgaris.
Two skin biopsies (punch biopsy, 4 mm) were taken in all cases, one each for routine histopathology and direct immunofluorescence (DIF).
Tissue samples that were obtained by biopsy were examined by routine histologic examinations, including hematoxylin and eosin staining and direct immunofluorescence examination.
Direct immunofluorescence microscopic examination of perilesional tissue showed linear deposits of IgA at the basement membrane zone (Figure 6).

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