In all patients, direct immunofluorescence
assay revealed IgG/C3 linear deposits along the BMZ.
(6.) Raghu AR, Nirmala NR, Sreekumaran N: Direct immunofluorescence
in oral lichen planus and oral lichenoid reactions.
Comparison of a commercial qualitative real-time RT-PCR kit with direct immunofluorescence
assay (DFA) and cell culture for detection of influenza A and B in children.
(13.) Thomas EE, Book LE: Comparison of two rapid methods for detection of respiratory syncytial virus (RSV) (TestPack RSV and Ortho RSV ELISA) with direct immunofluorescence
and virus isolation for the diagnosis of pediatric RSV infection, J Clin Microbiol 1991; 29: 632-5.
consists of the identification of elementary corpuscles in endocervical material, through fluorescent monoclonal antibodies, identified with an optical microscope [9,10].
(3) The sine qua non of the disease, though, is demonstration through direct immunofluorescence
of complement deposition and IgG in a linear band along the basement membrane.
Fecal positive samples were examined for Cryptosporidium and Giardia cysts by direct immunofluorescence
staining technique, using a commercial kit (Meriflour-Meridian Diagnostics, Inc., Cincinnati, Ohio).
Skin biopsies were performed, including for direct immunofluorescence
Keywords: Direct immunofluorescence
, Tzanck smear, pemphigus vulgaris.
Two skin biopsies (punch biopsy, 4 mm) were taken in all cases, one each for routine histopathology and direct immunofluorescence
Tissue samples that were obtained by biopsy were examined by routine histologic examinations, including hematoxylin and eosin staining and direct immunofluorescence
microscopic examination of perilesional tissue showed linear deposits of IgA at the basement membrane zone (Figure 6).