fluorescent antibody technique

(redirected from Direct Fluorescent Antibody Test)
Also found in: Acronyms.

fluor·es·cent an·ti·bod·y tech·nique

a technique for antigen using a fluorescent antibody, usually performed by one of two methods: direct, immunoglobulin (antibody) conjugated with a fluorescent dye is added to tissue and combines with specific antigen (microbe or other), the resulting antigen-antibody complex located by fluorescence microscopy; or indirect, unlabeled immunoglobulin (antibody) is added to tissue and combines with specific antigen, after which the antigen-antibody complex is labeled with fluorescein-conjugated antiimmunoglobulin antibody with the resulting triple complex located by fluorescence microscopy.

fluor·es·cent an·ti·bod·y tech·nique

(flōr-es'ĕnt an'ti-bod-ē tek-nēk')
A procedure to test for antigen with a fluorescent antibody by one of two methods: direct, in which immunoglobulin (antibody) conjugated with a fluorescent dye is added to tissue and combines with specific antigen (microbe, or other), the resulting antigen-antibody complex being located by fluorescence microscopy; or indirect, in which unlabeled immunoglobulin (antibody) is added to tissue and combines with specific antigen, after which the antigen-antibody complex may be labeled with fluorescein-conjugated anti-immunoglobulin antibody, the resulting triple complex then being located by fluorescence microscopy.

fluorescent antibody technique (FAT) or immunofluorescence

a technique used to show up the presence of a particular ANTIGEN, in which an antibody is labelled with a fluorescent dye (fluorochrome) that combines with the antigen and is subsequently located by its FLUORESCENCE.
References in periodicals archive ?
The direct fluorescent antibody test for detection of Brucella abortus in bovine abortion material.
Comparison of the rapid immunodiagnostic test (RIDT) to the direct fluorescent antibody test (DFAT, reference method) and murine neuroblastoma cell culture (MN) by species tested.
[ClickPress, Wed Jun 19 2019] Direct fluorescent antibody test kits detects the existence of an antigen (particularly a protein on the surface of a bacteria, virus or any other microbe).
[USPRwire, Tue May 28 2019] Direct fluorescent antibody test kits detects the existence of an antigen (particularly a protein on the surface of a bacteria, virus or any other microbe).
[ClickPress, Thu May 02 2019] Direct fluorescent antibody test kits detects the existence of an antigen (particularly a protein on the surface of a bacteria, virus or any other microbe).
Compared to the direct fluorescent antibody test, the traditional standard in rabies diagnosis, the dRIT was 100% sensitive and specific.
[UKPRwire, Thu May 02 2019] Direct fluorescent antibody test kits detects the existence of an antigen (particularly a protein on the surface of a bacteria, virus or any other microbe).
[ClickPress, Fri Apr 26 2019] Rapid visualization of bacteria from a sample such as a throat swab or sputum is achieved through direct fluorescent antibody test which drive the market of direct fluorescent antibody test kits.
Brains of animals that died during MIT were subjected to the direct fluorescent antibody test (DFAT) with polyclonal antirabies fluorescein (FITC)-labeled immune globulins (Scientific-Research Veterinary Institute, Kazan, Russia) or FITC-labeled antirabies diagnostic conjugate (Centocor Inc., Malvern, PA).
Samples sent to CDC for testing on December 21 included a nuchal skin biopsy, which tested positive for rabies virus by direct fluorescent antibody test on December 22, and saliva and skin, which were positive by reverse-transcriptase polymerase chain reaction (RT-PCR) assay on December 23.
Polymerase chain reaction and direct fluorescent antibody tests were inconclusive because of the deteriorated condition of the carcass.
Rapid methods such as polymerase chain reaction assays, direct fluorescent antibody tests, and viral cultures are now available.

Full browser ?