fluorescent antibody technique

(redirected from Direct Fluorescent Antibody Test)
Also found in: Acronyms.

fluor·es·cent an·ti·bod·y tech·nique

a technique for antigen using a fluorescent antibody, usually performed by one of two methods: direct, immunoglobulin (antibody) conjugated with a fluorescent dye is added to tissue and combines with specific antigen (microbe or other), the resulting antigen-antibody complex located by fluorescence microscopy; or indirect, unlabeled immunoglobulin (antibody) is added to tissue and combines with specific antigen, after which the antigen-antibody complex is labeled with fluorescein-conjugated antiimmunoglobulin antibody with the resulting triple complex located by fluorescence microscopy.

fluor·es·cent an·ti·bod·y tech·nique

(flōr-es'ĕnt an'ti-bod-ē tek-nēk')
A procedure to test for antigen with a fluorescent antibody by one of two methods: direct, in which immunoglobulin (antibody) conjugated with a fluorescent dye is added to tissue and combines with specific antigen (microbe, or other), the resulting antigen-antibody complex being located by fluorescence microscopy; or indirect, in which unlabeled immunoglobulin (antibody) is added to tissue and combines with specific antigen, after which the antigen-antibody complex may be labeled with fluorescein-conjugated anti-immunoglobulin antibody, the resulting triple complex then being located by fluorescence microscopy.

fluorescent antibody technique (FAT) or immunofluorescence

a technique used to show up the presence of a particular ANTIGEN, in which an antibody is labelled with a fluorescent dye (fluorochrome) that combines with the antigen and is subsequently located by its FLUORESCENCE.
References in periodicals archive ?
Comparison of the rapid immunodiagnostic test (RIDT) to the direct fluorescent antibody test (DFAT, reference method) and murine neuroblastoma cell culture (MN) by species tested.
Samples sent to CDC for testing on December 21 included a nuchal skin biopsy, which tested positive for rabies virus by direct fluorescent antibody test on December 22, and saliva and skin, which were positive by reverse-transcriptase polymerase chain reaction (RT-PCR) assay on December 23.
Polymerase chain reaction and direct fluorescent antibody tests were inconclusive because of the deteriorated condition of the carcass.
Rapid methods such as polymerase chain reaction assays, direct fluorescent antibody tests, and viral cultures are now available.
Serologic tests of CSF and serum for anti-rabies virus antibody, PCR tests of saliva and a nuchal biopsy for the presence of rabies RNA, and direct fluorescent antibody tests of the nuchal biopsy for rabies virus were performed.
Biopsy can be helpful, but it is no longer the preferred method to confirm diagnosis since the advent of more rapid methods such as polymerase chain reaction assays, direct fluorescent antibody tests, and viral cultures.

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