digoxigenin


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di·gox·i·gen·in

(dī-joks'ĭ-jen-in),
The aglycon of digoxin that is joined by 3 moles of digitoxose to form the glycoside, digoxin.
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References in periodicals archive ?
The inner primers were designed with a digoxigenin or a fluorescein moiety at their 51 end; thus, the short products were labelled with digoxigenin for the SJNNV genotype or fluorescein for the RGNNV genotype.
Towards a Dual Lateral Flow Nanobiosensor for Simultaneous Detection of Virus Genotype-Specific PCR Products
The PCR solution and carbon particles conjugated with avidin were directly added to the nitrocellulose membrane with two test lines of immobilized antibodies specific for digoxigenin and fluorescein.
Nucleic immunoassay simultaneously detects Listeria, L. monocytogenes
For labeling of PCR products, the reaction of PCR was performed by dNTP mixture containing digoxigenin labeling mix (Digoxigenin dNTPs, Roche, Germany) with the same condition.
Detection of E. coli O157:H7 and Shigella dysenteriae toxins in clinical samples by PCR-ELISA
The synthesis of the digoxigenin labeled riboprobe and the hybridization reactions with the polyprobe were conducted as previously described (HERRANZ et al., 2005) with some modifications.
Simultaneous detection of four viruses affecting apple and pear by molecular hybridization using a polyprobe/Deteccao simultanea de quatro virus que infectam macieiras e pereiras por meio de hibridizacao molecular com uma polissonda
The prepared digoxigenin labeledcDNA probe was used in dot blot and tissue print hybridization assays.
MOLECULAR CHARACTERIZATION OF THE 16SR II GROUP OF PHYTOPLASMA ASSOCIATED WITH FABA BEAN (Vicia faba L.) IN SAUDI ARABIA
Apoptosis was evaluated by the in situ terminal-deoxynucleotidyl-transferase-mediated dUTP digoxigenin nick end labeling (TUNEL) assay.
Potential novel biomarkers for diabetic testicular damage in streptozotocin-induced diabetic rats: nerve growth factor beta and vascular endothelial growth factor
To quantify apoptosis, T24 xenograft sections were processed for in situ immunocytochemical localization of nuclei exhibiting DNA fragmentation using the technique of terminal deoxynucleotidyl transferase- (TdT-) mediated dUTP digoxigenin nick-end labeling (TUNEL) in conjunction with an apoptosis detection kit (In Situ Cell Death Detection Kit, POD).
Curcumin inhibits mitochondrial injury and apoptosis from the early stage in EAE mice
The digoxigenin tail-labelled PGRS (5' CGG CCG TTG CCG CCG TTG CCG CCG TTG CCG CCG 3') or DR (5' CCG AGA GGG GAC GGA AAC 3') probes were used for hybridization which was performed at 65[degrees] C using rolling bottle method (Table 2).
Extensive genetic diversity among clinical isolates of mycobacterium tuberculosis in Central province of Iran
Digoxigenin labelled probes were used at a concentration of 80 ng/100 [micro]L in hybridization buffer [Tris-HCl 0.02 M pH 7,5; NaCl 0.3 M; EDTA 0.01 M; DTT 0.1 M; formamide 50%; Denhardt's 1X; tRNA 100 [micro]g/mL; ss-DNA 100 [micro]g/mL] overnight at 50[degrees]C in a moist chamber.
The involvement of the androgen receptor in the secretion of the epididymal corpus in the lizard Podarcis sicula
Such probes are commonly labeled with the molecule digoxigenin (DIG), which normally occurs in the plant species Digitalis purpurea.
Ultrapure water drives RNA technologies: the use of ultrapure water in RNA research using in situ hybridization ensures contamination-free, reliable results
Sections were incubated in a digoxigenin antibody solution (500:1 dilution) containing 2.5 mL buffer 1 (100 mM Tris, 150 mM NaCl, 0.3% Triton X-100, 1% goat serum, pH 7.5) with 5 [micro]L of antidigoxigenin Fab fragments conjugated with alkaline phosphatase (750 U/mL) (Roche, Mannheim, Germany).
Divergent astrovirus associated with neurologic disease in cattle
Some of the useful plant drugs include vinblastine, vincristine, Taxol, Podophyllotoxin, camptothecin, digitoxigenin, gitoxigenin, digoxigenin, tubocurarine, morphine, codeine, aspirin, atropine, Pilocarpine, capsicum, Ellison, curcumin, artemesinin and ephedrine among others.
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