Two smears are made for each needle pass; 1 slide is immediately fixed in alcohol for Papanicolaou staining and the other is air-dried and stained with
Diff-Quik for rapid on-site assessment by the cytopathologist.
Some of the tumor cells are multilobulated to multinucleated with pleomorphic, hyperchromatic nuclei (
Diff-Quik stain x20).
(a) Arborizing papillary fragments with fibrovascular cores in a granular and cystic background (
Diff-Quik Stain, x200), (b) single cells with vacuolated cytoplasm (
Diff-Quik stain x1000), and (c) cell block showing tumor cells with prominent cribriform configuration and eosinophilic secretions (H and E, x200).
felis was detected in
Diff-Quik stained blood smears (Fig.
A fine-needle aspiration biopsy of the bony lesions was performed, and the samples were fixed in methanol and stained with
Diff-Quik for cytologic evaluation.
Detailed morphological characteristics were described using
Diff-Quik rapid staining.
With exception of tape preparations the slides are heat fixed and stained using
Diff-quik, Giemsa, Gram's or Methylene blue.
BAL was performed, and staining with
Diff-Quik (Siemens Healthcare Diagnostics Inc., Deerfield, IL, USA) and modified trichrome showed that the fluid samples were now positive for intracellular yeast-like forms.
Photomicrograph showing highly cellular cytology smears (
Diff-Quik, original magnification X100).
Fine needle aspiration and impression samples from cutaneous lesions were obtained, air dried and stained with
Diff-Quik, for cytologic evaluation.
The shell vials were incubated at 34[degrees]C and monitored daily by
Diff-Quik staining (Dade International Inc., Miami, FL, USA) for rickettsiae.
The FNA direct smears are prepared as both air-dried and alcohol-fixed methods and are most commonly stained using
Diff-Quik and/or Papanicolaou stains.