Two smears are made for each needle pass; 1 slide is immediately fixed in alcohol for Papanicolaou staining and the other is air-dried and stained with Diff-Quik
for rapid on-site assessment by the cytopathologist.
Some of the tumor cells are multilobulated to multinucleated with pleomorphic, hyperchromatic nuclei (Diff-Quik
(a) Arborizing papillary fragments with fibrovascular cores in a granular and cystic background (Diff-Quik
Stain, x200), (b) single cells with vacuolated cytoplasm (Diff-Quik
stain x1000), and (c) cell block showing tumor cells with prominent cribriform configuration and eosinophilic secretions (H and E, x200).
felis was detected in Diff-Quik
stained blood smears (Fig.
A fine-needle aspiration biopsy of the bony lesions was performed, and the samples were fixed in methanol and stained with Diff-Quik
for cytologic evaluation.
Detailed morphological characteristics were described using Diff-Quik
With exception of tape preparations the slides are heat fixed and stained using Diff-quik
, Giemsa, Gram's or Methylene blue.
BAL was performed, and staining with Diff-Quik
(Siemens Healthcare Diagnostics Inc., Deerfield, IL, USA) and modified trichrome showed that the fluid samples were now positive for intracellular yeast-like forms.
Photomicrograph showing highly cellular cytology smears (Diff-Quik
, original magnification X100).
Fine needle aspiration and impression samples from cutaneous lesions were obtained, air dried and stained with Diff-Quik
, for cytologic evaluation.
The shell vials were incubated at 34[degrees]C and monitored daily by Diff-Quik
staining (Dade International Inc., Miami, FL, USA) for rickettsiae.
The FNA direct smears are prepared as both air-dried and alcohol-fixed methods and are most commonly stained using Diff-Quik
and/or Papanicolaou stains.