The inhibitors used were aminoguanidine, acetylsalicylic acid (aspirin), diethylenetriaminepentaacetic acid
, [alpha]-methyl-4-[2-methyl-propyl]benzeneacetic acid (ibuprofen), acetaminophen (paracetamol), L-ascorbic acid (vitamin C) and [alpha]-tocopherol (vitamin E) all at a concentration of 25 mmol/l.
Gates, "Filtration fraction and its implications for radionuclide renography using diethylenetriaminepentaacetic acid
and mercaptoacetyltriglycine," Clinical Nuclear Medicine, vol.
naja kaouthia were obtained from Latoxan; [sup.125]I-labeled [alpha]-BuTx (specific activity, 8.2 TBq/mmol or 74 TBq/mmol) was from Amersham Pharmacia Biotech; diethylenetriaminepentaacetic acid
(DTPA) anhydride and Eu[(N[O.sub.3]).sub.3] were from Sigma-Aldrich; Sephadex G-25, CM-Sephadex C-50, and mono-Q 5 x 5 columns were from Pharmacia Biotech; octylSi300 (5 [micro]m) HPLC support was from Serva; antihuman IgG (QSwAHuIgG) was from Sevapharma; DELFIA enhancement solution was from Wallac Oy; TE671 cell culture was from Deutsche Sammlung von Mikroorganismen and Zellkulturen GmbH; and Torpedo marmorata electric organs were from the Station Biologique d'Arcachon.
The diethylenetriaminepentaacetic acid
(DTPA) conjugate of arabinogalactan was prepared as described previously (21).
The incubation was performed in 50 mmol/L Tris-HCI buffer, pH 7.75, containing, per liter, 9 g of NaCl, 0.5 g of Na[N.sub.3], 3.5 g of Tween 20, 0.5 g of bovine gamma globulin, 20 [micro]mol of diethylenetriaminepentaacetic acid
(DTPA), and 5 g of BSA.
99m]Tc-labeled diethylenetriaminepentaacetic acid
(DTPA), [sup.51]Cr-labeled EDTA, and [sup.125]I-labeled iothalamate, have been used as reliable measures of glomerular filtration but are costly, involve special specimen handling, and require radiation exposure.
Before use, the antibodies were dissolved in 100 [micro]L of water and diluted 1:1000 in assay buffer (50 mmol of Tris-HCl, 0.15 mmol of NaCl, 0.5 g of Na[N.sub.3], 5 g of bovine serum albumin, 10 mg of diethylenetriaminepentaacetic acid
, and 0.1 mL of Tween 40 per liter).