1 Cycle Sequencing Kit (Applied Biosystems, USA), comprising DNA polymerase, a mixture of deoxyribonucleotide triphosphates, and fluorochrome labeled dideoxynucleotide
Sanger Sequencing is known as the chain termination method or the dideoxynucleotide
method or the sequencing by synthesis method.
Cycle sequencing reactions were performed at the Genomics Core Facility, Huck Institute for the Life Sciences, Penn State University, using 3' Big-Dye-labeled dideoxynucleotide
triphosphates (v 3.
Gene Sequencing and Analysis: The purified amplicons were sequenced using fluorescent dideoxynucleotide
terminators in an ABI PRISM 3130 automated sequencer (Applied Biosystem, Inc, Foster City, CA).
(ddNTP) terminator SBE chemistry of SNaPshot
After hybridization, the primer is extended by the polymerase with dideoxynucleotide
terminators in the absence of dNTPs.
5 [micro]M dideoxynucleotide
triphosphate (dNTP) mix, and 10U RNase inhibitor (Invitrogen) in a volume of 20 [micro]L at 37[degrees]C for 1h.
Genomes were finished by using PCR and traditional dideoxynucleotide
sequencing (Sanger) to resolve gaps and repetitive and low coverage areas.
In our experience pyrosequencing is cost-effective in testing samples when compared to qPCR, standard PCR, and dideoxynucleotide
chain-terminating sequencing methods.
This partial actin gene fragment was sequenced by the dideoxynucleotide
method from Centre for Applied Molecular Biology (CAMB), Lahore, Pakistan.
DNA sequencing employs poisonous dideoxynucleotide
tri-phosphates (diNTPs) in a 1:1 ratio with the nonpoisonous dNTPs.
HIV RNA extraction, reverse transcription, amplification, dideoxynucleotide
sequencing, and algorithmic interpretation of the sequencing data are steps of: