Conditions were kept same as discussed above during diazotization
Indirect flow injection voltammetric determination of sulphanilamide by monitoring at a glassy carbon electrode the excess of nitrite remaining after its diazotization
has been reported by Fogg et a1 .
The absorbance of the chromophore formed during diazotization
of the nitrite with sulphanilamide and subsequent coupling with napthylethylene diamine is used as the marker for NO scavenging activity .
reaction was allowed to proceed for about 30 min, with occasional shaking.
As an alternative, a diazotization
method has been used to measure the bilirubin concentration in AF (4).
4]-N and cadmium reduction followed by diazotization
with sulfanilamide for N[O.
Synthesis of new Congo Red derivatives (6a-j) was achieved by diazotization
of Congo Red at low temperature followed by coupling with a number of different coupling partners 5a-j in basic medium.
Starting from available 2-nitroaniline, we accessed 3,8-dibromobenzo[c]cinnoline through a coupling reaction and a ring-forming diazotization
In methods that rely on reduction of nitrate to nitrite by a metal or nitrate reductase, with subsequent determination of nitrite by diazotization
[9, 12, 13], deproteinization is essential to eliminate artifactually high results in most plasma or serum samples [9, 13].
Usually, azocompounds are prepared by diazotization
of the aromatic amine in mineral acid at about 0degC followed by coupling with nucleophiles .
First, a tandem Ullmann coupling--reductive diazotization
afforded 2,9-dichlorobenzo[c]cinnoline in high yield.
Our results confirmed this effect, regardless of whether the Jendrassik-Grof diazotization
method or the Vitros method was used.