Larvae stained using the NADPH diaphorase
assay were initially fixed in a 2% glutaraldehyde, 1% formaldehyde solution in phosphate buffer pH 7.
Under appropriate regulation, their presence would allow for the observed increase in NADPH diaphorase
activity during larval development (6) and account for the strong NOS immunoreactivity observed in the cell bodies of the apical ganglion (7).
The following polymorphic enzyme systems were assayed in two running buffers as described in Ritland and Ganders (1987): isocitrate dehydrogenase, triphosphoisomerase, 6-phosphoglucose dehydrogenase, phosphoglucomutase, phosphoglucoisomerase, aconotase, aspartate aminotransferase, diaphorase
(2 loci) and esterase.
8] YA([sections]) 1 Dia4 Diaphorase
3 UBC681a 1750bp CCCCCGACT 4 UBC858b 1400bp [(TG).
5] reductase activity (diphospho pyridine nucleotide reduced-dependent diaphorase
activity--represented as international units per gram Hb) by the methemoglobin ferricyanide method (23).
The immature activity of the enzyme diaphorase
in their erythrocytes makes infants more prone to methemoglobinemia in such circumstances; nonetheless, as my colleagues and I recently showed, even in preterm infants, applying EMLA before heel pricking does not produce methemoglobinemia .
1970), adenylate kinase (TVB), a-glycerophosphate dehydrogenase (TC), glycero-3-phosphate dehydrogenase (TC), glucose phosphate isomerase (CT), homoserine dehydrogenase (TVB), malic enzyme (TC), peptidase (Val-Leu) (RW), phosphofructokinase (TC), superoxide dismutase (CT), diaphorase
(RW), glutamate dehydrogenase (TVB), peptidase (Leu-Gly-Gly) (RW), pyruvate kinase (TVB), and triose phosphate isomerase (TC).
Using the same experimental paradigm in two independent studies, we have found that nicotinamide adenine dinucleotide phosphate diaphorase
(NADPH)+ neurons are reduced about 10% (Fernandez et al.
It was observed recently that the administration of Ascaris sun extract (Asc) in infected mice increased NADPH diaphorase
activity and TNF-[alpha] levels in the early phase of BCG infection (Ferreira et al.
1986)]: acid phosphatase (ACP), alanine dehydrogenase (ALA), butyrate esterase (EST), [Beta]-hydroxybutyrate dehydrogenase (HBD), diaphorase
(DIA), fumarase (FUM), fructose-1,6-diphosphatase (F16), glucose-6-phosphate dehydrogenase (G-6), glutamic-oxalacetic transaminase (GOT), glyceraldehyde-3-phosphate dehydrogenase type 1 (GP1), isocitrate dehydrogenase (IDH), indophenol oxidase (IPO), leucine aminopeptidase (LAP), leucine tyrosine peptidase (PEP), malate dehydrogenase (MDH), malic enzyme (ME), phosphoglucose isomerase (PGI), phosphoglucomutase (PGM), 6-phosphogluconate dehydrogenase (6PGD), and shikimate dehydrogenase (SDH).
histochemistry indicated NOS activity in cells in the lower lip of the larval mouth, the preoral hood, the gut, and in the tube feet of the echinus rudiment.
An additional 19 enzymes (acid phosphatase, alcohol dehydrogenase, aldolase, catalase, diaphorase
, endopeptidase, esterase, formate dehydrogenase, fluorescent [Beta]-galactosidase, glucose-6-phosphate dehydrogenase, [Beta]-D-glucosidase, glutamate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, isocitrate dehydrogenase, lactate dehydrogenase, malic enzyme, peroxidase, phosphoglucomutase, and sorbitol dehydrogenase) were studied, but excluded from the analysis because of inconsistent activity.