diaminobenzidine


Also found in: Dictionary, Thesaurus, Acronyms, Encyclopedia.

diaminobenzidine

(dī″ă-mēn″ō-bĕn′zĭ-dēn″),

DAB

A chromogen, C12H14N4, used in immunocytochemistry to highlight peroxidase.
Mentioned in ?
References in periodicals archive ?
A brownish-red precipitate generated by polymerization of the hydrogen peroxide with nitro 3,3'- diaminobenzidine in the treated tissues was observed (Fig.
The second antibody was biotinylated goat antirabic serum (Vector, Burlingame, CA, USA) (1:250); visualisation was with nickel-enhanced diaminobenzidine (Ni-DAB) for Fos, and diaminobenzidine (DAB) for double-labelling of kisspeptin.
The second antibody was biotinylated goat antirabic serum (Vector, Burlingame, CA, USA) (1:250); visualisation was with nickel-enhanced diaminobenzidine (NiDAB) for Fos, and diaminobenzidine (DAB) for double-labelling of kisspeptin.
Specimens were then soaked in Tris buffer containing 0.02% diaminobenzidine and 0.02% hydrogen peroxide for 1 min to achieve color development.
1:250 dilution of sheep antirabbit antibody (Avicenna Research Institute) was then added for 45 minutes followed by Diaminobenzidine (DAB) (Roche, Germany).
After further washes, the membranes were developed in 4-chloro1-naphthol/3,3' diaminobenzidine (CN/DAB, Pierce).
Processed cells that were reactive were visualized by the presence of a dark brown precipitate when color was developed with diaminobenzidine (DAB).
Diaminobenzidine (DAB); if not available, use amido black
Following a series of washes, the peroxidase reaction was completed using diaminobenzidine with a nickel solution being added to achieve a black color.
staining was performed with bond polymer detection kit and ultra view 3,3'-diaminobenzidine (DAB) detection kit and the sections were developed with diaminobenzidine tetrahydrochloride and counterstained with hematoxylin.
Expression levels of leptin and LepRb were evaluated by online tool, ImmunoRatio (http://153.1.200.58:8080/immunoratio/), which calculates the percentage of positively stained area (labeling index) by using a color deconvolution algorithm for separating the staining components (diaminobenzidine and hematoxylin) and adaptive thresholding for area segmentation [26].