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Depurination of a TTA loop (A/AP) of A(htel-21) also reduced the structural stability of the native GQ.
Mechanistic studies on depurination and apurinic site chain breakage in oligodeoxyribonucleotides.
After electrophoresis, the DNA was partially hydrolyzed (15 min depurination in 0.25 M HCl; 30 min denaturation in 0.4 mol [L.sup.-1] NaOH) before transfer to Hybond [N.sup.+] membrane (Amersham Biosciences, Piscataway, NJ).
For Southern blotting, the gel was treated successively in 0.25 N HC1 for 10 minutes to allow partial depurination and cleavage of large fragments, in denaturation solution composed of 0.5 M NaOH, 1.5 M NaC1 for 30 minutes and in 0.5 M Tris-HC1 (pH 7.4) for 30 minutes.
If the environment is more acidic (low pH), acid depurination is promoted, and if it's more alkaline, hydrolysis of the sugar-phosphate backbone increases.
The quinone metabolites of 4-OH-[E.sub.2] can interact with DNA and, in turn, lead to depurination of DNA--a potential mutagenesis event (Cavalieri et al.
This depurination of the SR loop results in irreversible inhibition of protein synthesis at the translation step by impairing both the elongation factor (EF) 1-dependent binding of aminoacyl-tRNA and the GTP-dependent binding of EF-2 to the affected ribosome (Gessner and Irvin, 1980).
Sodium adducts and depurination products in the spectrum were minor and did not interfere with assignment of genotype.
Five micrograms of DNA were digested with the appropriate restriction enzyme, fractionated on a 0.8% (w/v) agarose gel, and pressure-blotted onto Hybond-N nylon membranes (Amersham Pharmacia Biotech, Inc., Baie d'Urfe, Quebec) with the PosiBlot apparatus (Stratagene, La Jolla, CA) after depurination, denaturation and neutralization of the DNA .
This depurination ultimately results in the inhibition of protein synthesis within intoxicated cells (Figure 1B).
Reduction of cation adduction of oligonucleotides (14-16); minimization of DNA degradation, including depurination (17-19); and selection of proper matrices (20-24) are important to obtain high sensitivity, and the delayed extraction technology (25) has reduced ion fragmentation and increased spectral resolution.
In water, autocatalytic processes through depurination can result in a completely degraded DNA after only a few weeks.
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