Thus, hospital length of stay is much shorter when compared to amphotericin B deoxycholate
regimen, which should be administered for a period of 14-20 days.
Injection lipolysis with a cocktail of phosphatidylcholine and deoxycholate
: an Indian experience.
Wistar rats Regular diet 100 mg/kg in sodium caprate, or sodium deoxycholate
, once i.g.
Amphotericin B Deoxycholate
. Amphotericin B deoxycholate
is also called "conventional" amphotericin B.
To isolate the bacterial colonies, the samples were cultured with the aid of a platinum wire loop on Petri dishes containing blood agar and XLDagar (Xylose Lysine Deoxycholate
agar), using the agar depletion technique.
For bile resistant analysis, fresh culture of KCC-32 was inoculated into the sterilized MRS broth containing bile salts such as 0.5% sodium deoxycholate
and 0.3% oxgall (DCA Sigma, St Louis, MO, USA) and incubated at 37degC.
5% sodium deoxycholate
, 10 mg/ml aprotinin, 10 mg/ml leupeptin, and 1 mM phenylmethylsulfonylfluoride.
For western blot procedures, hypothalamus from AEA-treated and control animals was homogenized (Heidolph homogenizer DIAX 600) in 500 [micro]l of RIPA buffer (25 mM Tris-HCl; pH 7.6; 150 mM NaCl; 1% sodium deoxycholate
; 0.1% SDS) in the presence of protease inhibitor cocktail (Sigma-Aldrich, USA, P2714).
Proteins were extracted from cell pellets using lysis buffer (1 M Tris HCl pH8, Triton X-100 1%, and Na deoxycholate
0.25%) with the addition of PMSF 1 mM.
The suspension was centrifuged and the pellet was resuspended and washed twice with 50 mM Tris-Cl pH 8.0,300 mM NaCl, 0.25% sodium deoxycholate
, and 5mM DTT (the second time without sodium deoxycholate
Treatment was initiated with a daily regimen of amphotericin B deoxycholate
, starting with escalating doses.
A loop full of culture from selenite cysteine broth culture was subcultured onto Xylose lysine deoxycholate
(XLD) agar (Oxoid, England) plate and incubated aerobically at 37[degrees]C for 24 hrs.