denaturation

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denaturation

 [de-na″chur-a´shun]
a change in the usual nature of a substance, as by the addition of methanol or acetone to alcohol to render it unfit for drinking, or the change in the physical properties of a substance, such as a protein or nucleic acid, caused by heat or certain chemicals that alter tertiary structure.
protein denaturation any nonproteolytic change in the chemistry, composition, or structure of a native protein that causes it to lose some or all of its unique or specific characteristics.
Miller-Keane Encyclopedia and Dictionary of Medicine, Nursing, and Allied Health, Seventh Edition. © 2003 by Saunders, an imprint of Elsevier, Inc. All rights reserved.

de·na·tur·a·tion

(dē-na'tyū-rā'shŭn),
The process of becoming denatured.
Farlex Partner Medical Dictionary © Farlex 2012

de·na·tur·a·tion

(dē-nā'chŭr-ā'shŭn)
The process of becoming denatured.
Medical Dictionary for the Health Professions and Nursing © Farlex 2012

denaturation

1. Alteration in the folding pattern of a protein by heat or chemical reaction from its physiological conformation to an inactive shape.
2. In the case of DNA or RNA the conversion from a double-stranded structure to a single stranded structure, usually by heating. This is an essential stage in the POLYMERASE CHAIN REACTION.
Collins Dictionary of Medicine © Robert M. Youngson 2004, 2005

denaturation

the breakdown of the bonds forming the quaternary, tertiary and secondary structures of PROTEINS and NUCLEIC ACIDS, a process that can be caused by various agents, such as excess heat, strong acids or alkalis, organic solvents and ultrasonic vibration. In some cases, denaturation is irreversible as in, for example, the heating of egg albumen to give solid egg white. When denaturation occurs in enzymes, they are inactivated, with major effects in living organisms. Denatured nucleic acids can often be returned to their original configuration in the process of renaturation.
Collins Dictionary of Biology, 3rd ed. © W. G. Hale, V. A. Saunders, J. P. Margham 2005
References in periodicals archive ?
It can also preventcell from denaturating, making cell membrane integrating and normal conjunction of cell margins in order to protect cells' normal morphous in intestinal tissue.
Molecular methods, like DGGE (Denaturating Gradient Gel Eletrophoresis) and 16S rRNA genes have been used to identify a diversity of fungi and bacteria in different sugarcane soil managements (DINI-ANDREOTE at al., 2010; PISA et al., 2011), but, these methods do not allow quantification of microbial biomass.
bovis (NC 317), under denaturating, reducing conditions, was performed by the method of Lammli [21] with recommended modifications [14].
The purity and integrity of total RNA were monitored by electrophoretic analysis on denaturating agarose gel; ultraviolet spectrophotometry (Biorad) was used for RNA yield evaluation.
PCR cycling conditions were 40 cycles with 60[degrees]C annealing/extension temperature for 60 seconds and 95[degrees]C denaturating temperature for 15 seconds.
Rapid and accurate denaturating high performance liquid chromatography protocol for the detection of alpha-l-iduronidase mutations causing muco polysaccharidosis type I.
Rapid genotyping of CTX-M extended-spectrum [beta]-lactamases by denaturating high-performance liquid chromatography.
The digested product was sized by electrophoresis on a 10 per cent non denaturating polyacrylamide gel and genotype was determined by examination of 0.2 per cent ethidium bromide stained gels under a UV transilluminator.
The amplification was performed with 1 min denaturating at 95[degrees]C followed by 35 cycles of 94, 52 and 72[degrees]C, 1 min each with a 2.5 mM MgCl2, using AmpliTaq Gold DNA polymerase (Applied Biosystems, USA) using species-specific nestin primers (Table 2).
Sequencing reactions consisted of an initial denaturation step (2 min, 92 [degrees]C) followed by 30 cycles (30 s, 95 [degrees]C, denaturating; 30 s, 50 [degrees]C, annealing; 1 min, 72 [degrees]C, elongation), performed with the Sequitherm EXCEL II DNA sequencing kit-LC (Epicentre Technologies).
The PCR cycle program consisted of an initial denaturating cycle of 95[degrees]C for 10min and 40 repetitions of cycles of 95[degrees]C for 10s and 60[degrees]C for 65s [15].
Heteroduplex formation was induced by denaturating PCR products at 95[degrees]C for 5 minutes followed by gradual re-annealing from 95[degrees]C to 23[degrees]C (room temperature) prior to analysis.