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Dinitro chloro benzene (DNCB) was used for induction of delayed type hypersensitivity. DNCB forms dinitrophenyl protein by interacting with the skin (Sajid et al., 2007).This protein is responsible for stimulating T-cells.
Delayed type hypersensitivity assay: Mice were divided into groups and treated accordingly for eight days.
Delayed type hypersensitivity: Delayed type hypersensitivity (DTH) was observed by measuring and comparing the skin thickness of left and right side of the abdomen of albino mice.
Cell mediated immunity plays a defensive role not only against infectious organisms but tissue grafting, tumor immunity, and delayed type hypersensitivity with the aid of T lymphocytes and lymphokines.
Delayed Type Hypersensitivity Test: Delayed- type hypersensitivity (DTH) reaction was noticed in this test in broilers of all the groups as described by Tiwary and Goel (1985).
For delayed type hypersensitivity reaction with 2, 4-Dinitrochlorobenzene (DNCB), five broilers from each group were taken.
There was classical delayed type hypersensitivity skin reaction.
Triterpenoids remarkably inhibited delayed type hypersensitivity reaction (DTH).
Effect of triterpenoids on delayed type hypersensitivity (DTH) reaction