primer

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prim·er

(prī'mĕr),
1. A molecule, which may be a small polymer, that initiates the synthesis of a larger structure. Synonym(s): starter
2. A pheromone that causes a long-term physiologic change.

primer

(prī′mər)
n.
Genetics A segment of DNA or RNA that is complementary to a given DNA sequence and that is needed to initiate replication by DNA polymerase.

primer

[prī′mər]
1 a short piece of DNA or RNA complementary to a given DNA sequence that acts as a point at which replication can proceed, as in a polymerase chain reaction.
2 a molecule, such as a small polymer, that induces the synthesis of a larger molecule.

primer

1. A short RNA sequence paired with one DNA strand that provides a free 3’-OH end on which synthesis of a deoxyribonycleotide chain can start.
2. The short nucleotide sequence used to start the POLYMERASE CHAIN REACTION (PCR) process.

primer

a short OLIGONUCLEOTIDE, which is required for the functioning of various POLYMERASES. In DNA REPLICATION, the primer is a short RNA sequence that anneals to the DNA TEMPLATE and is extended by DNA POLYMERASE. In the POLYMERASE CHAIN REACTION, the primer is a short oligonucleotide that initiates the PCR. In OLIGONUCLEOTIDE-DIRECTED MUTAGENESIS, the synthetic oligonucleotide used to introduce the mutation is a mutagenic primer that initiates DNA synthesis.

primer

oligonucleotide which is hydrogen-bonded to the template strand of DNA; required for the replication of DNA by polymerase.

random primer method
a method of labeling DNA in vitro.
primer walking
an orderly method for sequencing DNA which involves obtaining some sequence, then making an oligonucleotide primer to the end of the sequence obtained and using it as a primer for the next sequencing reaction and repeating this until the whole sequence is determined.
References in periodicals archive ?
In this project, the degenerate primers, PALFor1EcoR1 (5'GAAGATCTTCGVTTCTTGAATGABGGAATYTTTYGGNA-3') and PALRevBgIII (5'- CGGAATTCCGTTADCAHATNGGAAGAGGAGCACCA-3') successfully amplified the corresponding PAL promoter gene sequence in orchid (Dendrobium crumenatum).
Use of degenerate primers in the polymerase chain reaction to detect whitefly-transmitted geminiviruses.
By RT-PCR, a 284 bp product was amplified using the degenerate primers P5 and P6 based on the EST (BI390105).
Those for cob cloning are "universal" degenerate primers, and those for quantification are specific primers for N.
The consensus primers GP5/6 (and the extended version GP5+/6+) (19) and the degenerate primers MY09/11 (and the modified version PGMY09/11) (20) are the most widely used primers.
A guide to the design and use of mismatched and degenerate primers.
PCR using degenerate primers reported to amplify a conserved region of the coat protein of potyviruses amplified a fragment of 33 bp.
Dengue serotype specific degenerate primers designed according to the primer sequences targeting C-prM gene junction (Lanciotti et al.
A product of 432 bp was obtained by PCR amplification with degenerate primers and then sequenced.
We designed degenerate primers for nested PCR screening on the basis of a multiple sequence alignment of the NS1 gene from human BuV, WUHARV parvovirus and MpBuV as follows: BuV-F1 (position 190-215 in MpBuV genome, 5'-TCAAWRTMACCTGGAAAGACrACAGA-3') and BuV-R1 (position 1503 1534, 5'-TCATTGGTTGTCATKAYWACTGGAGTTGGTTC-3') for the first PCR round, and BuV-F2 (position 980-1006, containing an equimolar mixture of 5'-AGAAAAATGGATGCTCCAAGATCCAGA-3' and 5'-AGAAAAATGGATGCTTGGTGAWCCWGA-3') and BuV-R2 (position 1444 1465, 5'-ATTGCTTGGCCACTCATGATKG-3') for the second PCR round.
For difficult or unknown targets, a new strategy for barcoding primer design is to couple multiple degenerate primers into a single primer cocktail with strategic placement of the nucleoside inosine, which pairs with A, C, and T.