The SCs proliferation was promoted via miR-122/PDK4/ DUSP4 and miR-449a/FKBP1B pathways
In this study, miR-122 was found to target PDK4 and DUSP4. The PDK4 protein located in mitochondria and inhibiting the catalyzing function of pyruvate dehydrogenase (PDH) (Grassian et al., 2011).
The DUSP4 protein was essential in the process of endoderm generating spermatogonium or oogonium (Brown et al., 2008).
In our results, The SCs was inhibited by DUSP4, PDK4 or FKBP1B overexpression.
The comparison analysis was done on miR-122, miR-449a, DUSP4, PDK4 and FKBP1B expression levels in 1-day, 12-months-old and 24-months-old bull testes tissues.
In conclusion, meanwhile, PDK4 and DUSP4 were the target genes of miR-122, FKBP1B was the target gene of miR-449a.
Transcriptional profiling of endogenous germ layer precursor cells identifies dusp4 as an essential gene in zebrafish endoderm specification.