NADPH dehydrogenase (quinone)

(redirected from DT-diaphorase)

NADPH de·hy·dro·gen·ase (quin·one)

a flavoprotein that oxidizes NADH or NADPH to NAD+ or NADP+ with quinones (for example, menadione) as hydrogen acceptors.
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References in periodicals archive ?
Qapzola is activated by DT-diaphorase, an enzyme over-expressed in bladder cancer cells, to generate cytotoxic species leading to cell death.
Myocardial antioxidants including reduced glutathion(GSH), glutathione-S -transferase (GST), and DT-diaphorase activities were reduced ,while lipid peroxidation was increased.
Rats were sacrificed after ECG, hearts were removed, homogenized and stored for subsequent measurement of lipid peroxides, glutathione (GSH) contents, glutathione-S-transferase (GST), and DT-diaphorase activities.
The activity of DT-diaphorase was assayed as described by Benson et al.
The resulting supernatant (cytosolic fraction) was used for assaying total cytosolic GSH S-transferase (GST), DT-diaphorase (DTD), Lactate dehydrogenase (LDH) and antioxidant enzymes.
Determination of glutathione S-transferase and DT-diaphorase activity
The gene functions of this cluster are diverse containing among others, Ly6d (lymphocyte antigen 6 complex), a lymphocytic marker; Cstb (cathespin), an intracellular thiol inhibitor; Nqo1 (DT-diaphorase) a two-electron quinone oxidoreductase whose expression is often associated with redox stress; and another member of the annexin family, others of which are in the cluster shown in Figure 3A; and Krt8 (Keratin 8), commonly associated with Mallory body formation.
Apaziquone is a novel anticancer drug that is activated, to become a cytotoxic alkylating agent, through bio-reductive enzymes, such as DT-diaphorase, that are over-expressed in bladder cancer cells.
The hepatic glutathione S-transferase and DT-diaphorase specific activities were elevated above basal level by basil leaf treatment (from p < 0.005 to p < 0.001).
The supernatant (cytosol fraction), after discarding any floating lipid layer and appropriate dilution, was used for the assay of glutathione S-transferase, DT-diaphorase, lactate dehydrogenase and antioxidant enzymes, whereas the pellet representing microsomes was suspended in homogenizing buffer and used for assaying cytochrome [P.sub.450], cytochrome [b.sub.5], cytochrome [P.sub.450] reductase, cytochrome [b.sub.5] reductase and lipid peroxidation.
A modulatory effect of two doses and BHA was also observed for the activities of glutathione S-transferase, DT-diaphorase, superoxide dismutase and catalase in the kidney, lung and forestomach, as compared with the control group.
3.06 [+ or -] 0.39 (b) (100 mg/kg body wt) (1.15) * IV BHA 3.56 [+ or -] 0.12 (c) (0.75% in diet) (1.34) ** The statistical analysis according to control values: (p < 0.05) (b); (p < 0.001) (c); (p < 0.005) (d) * compared to control values; (#) Not significant; A -- [micro]mole GSH formed/g tissue; B -- [micro]mole CDNB-GSH conjugate formed/min/mg protein; C -- nmole DCPIP reduced/min/mg protein; T-SH -- total sulfhydryl; NP-SH -- nonprotein sulfhydryl; PB-SH -- protein-bound sulfhydryl groups; GST -- glutathione S-transferase; DTD -- DT-diaphorase Table 4.