and DMP-1 have been suggested to be the main phenotypic markers of odontoblasts .
Genes Sequence Size (bp) Forward: 5 '-ACCCGCTGTCTTCTAGCGT-3' BMP2 Reverse: 5'-TTTCAGGCCGAACATGCTGAG-3' 180 Forward: 5,-CCTCCTGGTATCTCAACCACA-3' Foxc2 Reverse: 5'-GAGGGTCGAGTTCTCAATCCC-3' 134 Forward: 5'-CTATCCTGGCTCCGTGCTC-3' ALP Reverse: 5,-GCTGGCAGTGGTCAGATGTT-3' 100 Forward: 5'-CTCACACTCCTCGCCCTATT-3' OCN Reverse: 5'-TTGGACACAAAGGCTGCAC-3' 107 Forward: 5'-GCCACTTTCAGTCTTCAAAGAGA-3' DSPP
Reverse: 5'-GCCCAAATGCAAAAATATGTAA-3' 130 Forward: 5,-AAAATTCTTTGTGAACTACGGAGG-3' DMP1 Reverse: 5'-GAGCACAGGATAATCCCCAA-3' 94 Forward: 5'-AAGGTGAAGGTCGGAGTCAA-3' GAPDH Reverse: 5'-AATGAAGGGGTCATTGATGG-3' 108
The level of DSPP
, ALP, and OCN mRNAs increased in a time dependent manner.
Shares for a DSPP
can come from a new issue, from those held in treasury, perhaps from a buyback, or through open market purchases.
The mRNA expression levels of DSPP
, DMP-1, Runx2, and OCN were determined using qRT-PCR.
Interestingly, the expression level of dentin-specific marker DSPP
was highly detected in DDM/hDPSCs transplant, but not in HA-TCP/hDPSCs transplant (Figure 7(e)), indicating that DDM might have a better effect on dentin regeneration than HA-TCP.
Further, the late-stage gene expression analysis in Figure 7 revealed that Npnt tended to direct the hDPSCs' differentiation toward osteoblastic lineage instead of odontoblastic lineage, as the DSPP
, a well-established odontoblast differentiation marker , was moderately suppressed by Npnt, but BSP, a widely recognized osteoblast marker,  was markedly enhanced by Npnt compared to the other groups.
primers were sense, 5'-GCATTTGGGCAGTAGCATGG-3'; and antisense, 5'-CTGACACATTTGATCTTGCTAGGAG-3'.
Kim, "Functional splicing assay of DSPP
mutations in hereditary dentin defects," Oral Diseases, vol.
Odontoblasts secrete several collagenous and noncollagenous proteins, such as type I collagen, osteopontin, dentin matrix protein 1 (DMP1), and dentin sialophosphoprotein (DSPP
), which are special biological markers for the odontoblast/osteoblast-like differentiation of DPCs [15, 16].