Another treatment of adipose tissue involved a five-day process of multiple freeze-thaw cycles and alternating between two different enzymatic digestion solutions (one containing trypsin and the other containing DNase II
, RNase III, and lipase IV), in addition to polar solvent extractions .
This DNase is more active on double-stranded DNA than on single-stranded DNA and differs from the other three types of endonucleases that are involved in DNA fragmentation (DNase I, DNase II
, and NUC 18) .
Nuclear translocation of DNase II
and acid phosphatase during radiation-induced apoptosis in HL60 cells.
PO may be similar to DNase II
, which functions in eliminating DNA from dead cells or non-self DNA in vivo.
The contents of apoptotic cells are rapidly ingested by professional phagocytes or neighboring cells through mechanisms that are not fully understood (4), and the DNA is consequently completely digested by DNase II
in lysosomes (1).
An exciting new observation is that cell-free DNA itself may be directly implicated in the etiology of RA, as suggested by a recent study on DNase II
knockout mice, in which the mice developed symmetrical, erosive polyarthritis (34).