Nizet says that his team's discovery could guide a new approach to fighting disease, although researchers will first need to find ways to target only pathogenic DNases
. Rather than killing the bacteria with antibiotics, and thereby encouraging them to evolve resistance, he says, "we're basically allowing the immune system to do its job."
A vital requirement for this technique is a water source free of detectable levels of DNA and DNase
the partners recently launched a joint venture to market DNase
in Europe, Japan and Latin America.
In the second experiment, the mice were randomly assigned to two groups, the vehicle group (n = 10) and the DNase
group (n = 10).
production was done on DNase
agar (Difco) reconstituted according to the manufacturer's instruction and following the procedures as described by Collins and Lyne (10).
Manufactured from extractables-free polypropylene in an ISO 10,000 approved cleanroom environment, the low-profile plates undergo extensive quality testing to ensure they are of the highest quality, certified free from DNase
, RNase and endotoxin contamination.
RNA aliquots were treated with RNase-Free DNase
(Qiagen) in accordance with the manufacturer's protocol.
Similar experiments used DNase
to hydrolyze extracellular DNA during biofilm formation.
Produced in Class 10,000 clean room conditions, the Porvair range of PCR plates are certified free of pyrogens as well as DNAse
and RNAse enzyme activity enabling routine achievement of excellent PCR results.
Most of the isolated bacteria in this research have had the capability to produce DNase
Biochemical properties were confirmed by Gram staining, mannitol fermentation, catalase and DNase
Viral RNA was isolated by the QIAamp Viral RNA Mini Kit (QIAGEN, Hilden, Germany), treated with Dnase
I (Invitrogen, Carlsbad, CA, USA), and reverse transcribed to cDNA with Superscript II reverse transcriptase (Invitrogen).