The added sequence will bind up the messenger RNA with the same powerful bonding that holds together a DNA double helix, thus creating a freak double-stranded RNA or a DNA-RNA hybrid.
We have seen reductions of the specific product proteins as well as observed the expected DNA-RNA hybrids to show how the antisense is working," says Eric Wickstrom of the University of South Florida in Tampa.
Different studies suggest that transcription-associated recombination (TAR) is in large part due to collisions between transcription and replication, but increasing evidence indicate that R-loops, formed by a DNA-RNA hybrid
and a displaced single-stranded DNA, may be a major determinant of genome instability.
Such a DNA-RNA hybrid
would be resistant to both RNase and DNase activity.
The professor of genetics, Andres Aguilera, head of the Molecular Biology Department of Cabimer and Department of Genetics of the University of Seville, received the 13th Javier Benjumea Puigcerver Research Prize for the extraordinary scientific value of his work entitled DNA-RNA hybrids
as cause of genetic instability associated with tumours.
Another point that has to be taken into account is that the used antibody can react with different DNA-RNA hybrids
with different affinity (24), influencing the sensitivity of the assay for this miRNA and requiring additional calibration.
The H stands for hybrid, and this enzymatic activity degrades away the RNA pair of annealed DNA-RNA hybrids
In each case, specific HPV DNA-RNA hybrids
Specificity, however, may be further improved because biotinylated oligonucleotides specific for HPV DNA sequences are used to capture HPV DNA-RNA hybrids
(instead of anti-DNA-RNA polyclonal antibody used previously) onto streptavidine-coated wells of a microtiter plate.