DNA sequencing

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Related to DNA sequencing: DNA fingerprinting

DNA sequencing

The determination of the sequence of nucleotides in a sample of DNA.

base-sequence analysis

A generally automated method for determining the order of nucleotide bases in a nucleic acid (DNA or RNA).

DNA sequencing

The determination of the sequence of base pairs in a length of DNA.

DNA sequencing

determination of the order of NUCLEOTIDES in a DNA molecule or part thereof. A number of techniques is available to determine a DNA sequence, for example the chain-terminator DNA sequencing procedure (see DIDEOXYRIBONUCLEOTIDE). Generally such techniques rely on the power of polyacrylamide gels to resolve NUCLEIC ACID molecules that differ in length by only a single nucleotide. Ladders of DNA fragments are formed on the gels following ELECTROPHORESIS. Both manual and automated methods of sequencing are available.

References in periodicals archive ?

elPrep is designed as a multi-threaded application that runs entirely in memory, avoids repeated file I/O, and merges the computation of data of several DNA sequencing preparation steps.

ELPREP SPEEDS UP GENOME SEQUENCING ANALYSIS

There is no denying the promising future of DNA sequencing in the clinical laboratory.

DNA Sequencing in the Clinical Laboratory: A Ladder to the Future

The company's sequencing platform is expected to enable unique scientific discovery across the world's most complex diseases, accelerate critical areas of novel scientific discovery, and complement in a very powerful manner existing short-read DNA sequencing technologies.

ZS Genetics, Hitachi High Technologies America to Co-Develop Single-Molecule DNA Sequencing Platform

The upstream sectors of DNA sequencing industry are extremely robust, with the global market dominated by the second-generation DNA sequencing enterprises including Illumina, Thermo Fisher Scientific (through Life Technologies), Roche and the third-generation sequencing enterprises such as Pacific Biosciences.

DNA sequencing market to hit $10 billion by 2017

Companies profiled in "Global and China DNA Sequencing Industry Research Report, 20142017" include Illumina, Thermo Fisher Scientific, BGI, Roche, Qiagen, Pacific Biosciences, Sequenom, DA AN Gene, Agilent Technologies, Berry Genomics, Hunan China Sun Pharmaceutical Machinery and Jilin Zixin Pharmaceutical Industrial.

DNA sequencing market to hit $10 billion by 2017

The NEXTflex[TM] PCR-Free DNA Sequencing Kit features "Enhanced Adapter Ligation Technology" which results in library preps with a larger number of unique sequencing reads.

Bioo Scientific Launched the NEXTflex[TM] PCR-Free DNA Sequencing Kit Reducing Bias in NGS Library Prep

Capillary DNA sequencing: maximizing the sequence output.

Modified plasmid DNA preparation for capillary automated DNA sequencing

"I believe that it will take three to five years to bring cheap DNA sequencing to the medical marketplace, assuming an aggressive research and development program is in place," said Meller.

New method to make DNA sequencing faster and cheaper

For the past several years, the speed of DNA sequencing and the amount of DNA sequence that can reasonably be mapped in one experiment has gradually and methodically increased.

Personalized genome around the corner?

Bartonella ITS and pap31 DNA sequencing was performed for 2 positive amplicons for both the ITS and pap31 gene to confirm the Bartonella species and strain.

Bartonella henselae in porpoise blood

Contributors from the biomedical side and the technical side discuss the different analytical DNA sequencing techniques that have been developed and used since the practice began in 1977, and some of its applications.

Analytical techniques in DNA sequencing

A number of strategies have been used to identify SNPs, and of these, DNA sequencing has become the dominant technology.