DNA polymerase


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Related to DNA polymerase: RNA polymerase, DNA replication

nu·cle·o·tid·yl·trans·fer·as·es

(nū'klē-ō-tī'dĭl-trans'fĕr-ās'ĕz),
Enzymes that catalyze the transfer of transferring nucleotide residues (nucleotidyls) from nucleoside di- or triphosphates into dimer or polymer forms. Some nucleotidyltransferases bear specific names (for example, adenylyltransferases), trivial names indicating the linkage hydrolyzed in the synthesis (pyrophosphorylases, phosphorylases), or names indicating the material that is synthesized (for example, RNA or DNA polymerase).

DNA polymerase

n.
Any of various enzymes that function in the replication and repair of DNA by catalyzing the linking of dATP, dCTP, dGTP, and dTTP in a specific order, using single-stranded DNA as a template.

DNA polymerase

a group of enzymes that are responsible for the polymerization of free NUCLEOTIDES on to the unwound DNA molecule during DNA REPLICATION. The first DNA polymerase was discovered by A. Kornberg in 1957 (for which work he received the Nobel Prize). There are several polymerases in any organism. For example, in E. coli, DNA polymerase III synthesizes both the leading and the lagging strands (see DNA, REPLICATION); DNA polymerase I fills in the gaps between Okazaki fragments. In EUKARYOTES, three DNA polymerases are involved in chromosome replication: polymerase α, δ and ɛ; mitochondrial DNA synthesis involves polymerase γ. Most DNA polymerases also have an EDITING function.
References in periodicals archive ?
For the second question (use of proofreading enzymes), 18 laboratories replied that they did not use a DNA polymerase with 3'-5' exonuclease activity; 11 of these had reported homozygous results, 6 had reported heterozygous results, and 1 did not report a numeric result (only normal).
Bakuchiol (1), belonging to meroterpene class of natural product, has been reported as the strongest DNA polymerase [alpha] inhibitor, found in P.
Structures of mismatch replication errors observed in a DNA polymerase. Cell 2004; 116:803-16.
Taq DNA polymerase is isolated from the thermophilic bacteria, Thermophilus aquaticus, and has a stable natural structure.
The nucleotide incorporation reaction efficiency also depended on the DNA polymerase. Two different DNA polymerases were tested in this experiment: exo- Klenow and Sequenase version 2.0 T7 DNA polymerase.
Caption: Figure 3: Effect of different concentrations of Taq DNA polymerase on the performance of thermostabilized multiplex PCR.
As an enzyme for PCR, we used the following types of DNA-dependent DNA polymerases: Taq DNA polymerase, Tersus DNA polymerase, Deep Vent DNA polymerase and Phusion DNA polymerase.
In the paper, according to RSAS, "Modrich demonstrated the requirement of DNA polymerase III, exo-nuclease I, and DNA ligase for mismatch repair.
For method validation purposes, we used Taq DNA polymerase, a Family A DNA polymerase and the enzyme used in the earliest PCR experiments [6].
The concentrations used for each DNA polymerase are specified in the respective figure legends.
However, researchers have been trying to produce such enzyme from various versions of Taq DNA polymerase gene via genetic engineering.

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