They will hybridize next to each other and form a substrate for DNA ligase
activity only when a perfect match occurs, and are particularly sensitive to disruption from any mismatch near the nick--like a single nucleotide polymorphism (SNP).
Jackson, "Mammalian DNA double-strand break repair protein XRCC4 interacts with DNA ligase
IV"' Current Biology, vol.
In the paper, according to RSAS, "Modrich demonstrated the requirement of DNA polymerase III, exo-nuclease I, and DNA ligase
for mismatch repair.
(1997) Mammalian DNA double-strand break repair protein XRCC4 interacts with DNA ligase
Hura et al., "A flexible interface between DNA ligase
and PCNA supports conformational switching and efficient ligation of DNA," Molecular Cell, vol.
The total volume of the LDR reaction system was 10 [micro]l including 1 [micro]l 10 x Buffer, 1 [micro]l primer and probe mixture (0.5 pM/each), 0.05 [micro]l (40 U/[micro]l) ligase (Taq DNA ligase
enzyme system), 1 [micro]l PCR product (100 ng/[micro]l), and 6.95 [micro]l deionized water.
The kit combines TurboLigase[TM], an ultrapure recombinant T4 DNA Ligase
, and 5x TurboLigation buffer, an optimized quick ligation buffer.
The amplified gene of cellulase was purified from the agarose gel after electrophoresis using Qiagen gel extraction kit and ligated into the pTZ57R/T cloning vector (Fermentas) using DNA ligase
and ligation buffer.
LDR reactions were carried out in a volume of 20 [micro]L containing 20 mmol/L Tris-HCl (pH 7.6), 10 mmol/L Mg[Cl.sub.2], 100 mmol/L KCl, 10 mmol/l DTT, 1 mmol/L EDTA, 1 mmol/L NAD+, 12.5 mmol/L each probe, 3[micro]L of PCR product, and 0.1 mmol/L DNA ligase
. The LDR was performed in a PE 9600 thermal cycler by incubating at 95[degrees]C for 2 min and cycling for 30 cycles at 95oC for 30 s and 65[degrees]C for 4 min.
Descriptively, the hOGG1 gene on chromosome 3p26 catalyzes the cleavage of a glycosylic bond between the modified base and a sugar moiety, leaving an abasic apurinic/apyrimidinic site in DNA which is then incised followed by a few successive actions of a phosphodiesterase that completes the repair process with a final function by a DNA polymerase, and a DNA ligase
Note exquisite sensitivity in cell strains derived from DNA damage sensing- or repair-deficient cells derived from patients with abnormal ATM, NBS1 and DNA ligase
In the blunt end, a DNA ligase
will join together the 3' end and the 5' end of one molecule with the 5' end and the 3' end, respectively, of another molecule.