Evaluation of apoptosis using DNA laddering
: After 14 days of culture, the human ovarian tissues and apoptosis-induced tissues were assessed for DNA laddering
(n=3 in each subgroup).
Those were used for DNA laddering
assay and calculation of oxidative stress parameters.
Meanwhile, early and late apoptotic events via the Annexin V-FITC and DNA laddering
Assessment of DNA damage in bone marrow mesenchymal stem cells following Imidazole treatment by fast halo assay and DNA laddering
assay is a common method to detect apoptotic DNA fragmentation in cells.
Detection of DNA laddering
in influenza virus infected HeLa cells: DNA laddering
is commonly accepted as an important biochemical hallmark of apoptosis.
This study was undertaken to determine if internucleosomal DNA cleavage (DNA laddering
) and caspase-dependent PCD occurs in apoptotic neurons.
Thus, although present in a small percentage of cells, DNA laddering
from apoptotic cells was well below the detection limit of our biochemical method.
Apoptosis-related DNA laddering
was visible after treatment of MCF-7 cells with increasing CSA concentrations for 48 h (Fig.
Apoptotic phenotypes including apoptotic body formation, DNA laddering
, caspase 3 activation and cleavage of PARP proteins were induced by [CM.sub.FB] treatment but only slightly induced by same concentration of [CM.sub.MY] treatment in human HL-60 leukemia cells.
The occurrence of apoptosis mediated cell death was confirmed through the activation endonucleases-mediated nucleosome excision leading to the observation of DNA laddering
of about 180-200 bp.