Meanwhile, early and late apoptotic events via the Annexin V-FITC and DNA laddering
Tenders are invited for DNA Laddering
Kit (24) and 28 Other Items.
Assessment of DNA damage in bone marrow mesenchymal stem cells following Imidazole treatment by fast halo assay and DNA laddering
Detection of DNA laddering
in influenza virus infected HeLa cells: DNA laddering
is commonly accepted as an important biochemical hallmark of apoptosis.
Following MAP treatment, apoptotic thymocytes showed TUNEL and CMI antibody positivity, DNA laddering
and a 25-fold increase of DEVD-AFC cleavage (1.
Thus, although present in a small percentage of cells, DNA laddering
from apoptotic cells was well below the detection limit of our biochemical method.
Apoptosis-related DNA laddering
was visible after treatment of MCF-7 cells with increasing CSA concentrations for 48 h (Fig.
Apoptotic phenotypes including apoptotic body formation, DNA laddering
, caspase 3 activation and cleavage of PARP proteins were induced by [CM.
To confirm these observations, A549/DDP cells apoptosis was also monitored by transmission electron microscopy and DNA laddering
The occurrence of apoptosis mediated cell death was confirmed through the activation endonucleases-mediated nucleosome excision leading to the observation of DNA laddering
of about 180-200 bp.
The DNA laddering
was documented using an UV-transilluminator with CCD camera (UVBioDoc-It [TM] System, al-Biotech GmbH, Martinried, Germany).
However, administration of Coptidis Rhizoma extract prevented the DNA laddering
caused by ischemia-reperfusion.