The 100bp DNA ladder
was used as a molecular marker to measure the size of amplified DNA band (Invitrogen).
M: 2-log DNA ladder
(0.1 to 10.0 kb) (New England Biolabs, Ipswich, MA, USA).
Lanes 1-4, pools of Anopheles sundaicus mosquitoes; lane 5, size marker DNA (100-bp DNA ladder
); lanes 6 and 7, An.
1= Ses17; 2-Ses28; M= 1kb DNA ladder
; 3=Ses7; 4=Ses18; 5=Ses32; 6=Ses2; 7=Ses13; and 8= Ses20
The PCR products were separated on 1.2% agarose gel (Fermentas) stained with GelRed (Botium), using the GeneRuler 100pb DNA Ladder
(Life Technologies) as size marker.
Caption: Figure 1: Representative agarose gel image showing nucleotide polymorphism by polymerase chain reaction (PCR) restriction fragment length polymorphism of (a) xeroderma pigmentosum complementation group D (XPD) C22541A at codon 156 of the exon 6 (lane 1: 100bp DNA ladder
, lane 2: Uncut PCR product, lane 3: Wild-type allele, lane 4: Heterozygote, and lane 5: Variant allele), (b) XPD G23592A at codon 312 in the exon 10 (Lane 1: 100bp DNA ladder
, lane 2: Uncut PCR product, lane 3: Asp/Asp genotype lane 4: Asp/Asn genotype, and lane 5: Cys/Cys genotype), and (c) XPD A35931C at codon 751 in the exon 23 (lane 1: 100bp DNA ladder
, lane 2: Uncut PCR product, lane 3: Lys/Lys genotype lane 4: Lys/Gln genotype, and lane 5: Gln/Gln genotype)
The entire Pfu DNA polymerase, 100 bp DNA ladder
, 1 Kb DNA ladder
, protein size marker, restriction endonucleases, and T4 DNA ligase (Thermo Scientific Life Sciences GmbH, Hilden, Germany) monoclonal anti-polyhistidine peroxidase conjugate (Sigma-Aldrich Corp., St.
M: DNA ladder
(10Kbp), visualized under gel documentation system, Lane (1, 2, 3, 4, 5) SGP from tissue samples, Lane (6, 7, 8, 9) SGP from blood samples, NC negative control.
Total DNA was extracted from the tissues separately, according to the instruction of the kit (Apoptotic DNA Ladder
Kit 1 835 246 Roche, Germany).
All the samples were loaded into the wells of submarine gel while in first well DNA ladder
(Vivantis, UK product 3000bp) was loaded.
A 100 bp DNA ladder
(Norgen PCR Sizer 100 bp DNA Ladder
) loaded along with the gels was used for band sizing.