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The PCR products were checked on 2% agarose gel by doing electrophoresis for 90 min at 105 volts along with DNA ladder
After staining, the bands were visualised under ultraviolet (UV) light and different mutations were analysed with the sizes of PCR-amplified product and estimated according to the migration pattern of a 100-bp DNA ladder
mutations of superoxide dismutase gene.
Gene Ruler DNA ladder
100 bp was employed for estimating the rough DNA size range.
of 100 bp (Norgen, Canda) was used to determine the size of the amplified bands.
Caption: Figure 1: Agarose gel image showing nucleotide polymorphism by PCR-RFLP of (A) hOGG1 codon326 in the exon-7 (Lane 1: 100bp DNA ladder
, lane 2: Uncut PCR product, lane 3: Ser/Ser genotype lane 4: Ser/Cys genotype, lane 5: Cys/Cys genotype and) and (B) APE1 codon 148 in the exon-5.
The sizes of PCR products were estimated according to the migration pattern of a 100-bp DNA ladder
(Fermentas Technologies USA).
5 X TBE buffer and visualized by ethidium bromide staining under UV illumination (UVP model M20); their sizes were estimated by comparison with O Gene Ruler 1kb Plus DNA ladder
Together with the PCR products, a molecular weight pattern was applied to the gel (2Log DNA Ladder
or 100bp DNA Ladder
A DNA ladder
was formed at 48 h, indicating DNA fragmentation in the final phase of apoptosis.
The amplification product which included the 16S rRNA gene region plus the flanking regions when subjected to electrophoresis against 1 kb DNA ladder
appeared to be around 1861 bp which was confirmed by sequencing.
The fragment sizes were detected by comparing the amplicons with a 100 bp DNA Ladder