A large number of systems based on DNA hybridization
detection have been currently developed.
Man et al., "Real-time reliable determination of binding kinetics of DNA hybridization
using a multi-channel graphene biosensor," Nature Communications, vol.
Zhao, "Kinetics and thermodynamics of DNA hybridization
on gold nanoparticles," Nucleic Acids Research, vol.
When DNA hybridization
is detected electrically, CNT nano-bioconjugates for DNA detection are deposited on electrode surfaces.
Another moderately rapid test is the Affirm VPIII Microbial Identification Test (Becton Dickinson; Sparks, MD), which relies on direct DNA hybridization
. This test does not "amplify" the DNA present so it is not as sensitive as the newest types of diagnostic tests cleared by the FDA (see nucleic acid amplification tests [NAATs], below).
and Maeda, M., Rapid aggregation of gold nanoparticles induced by non-cross-linking DNA hybridization
. Journal of the American Chemical Society, 2003; 125(27): 8102-8103.
The nine papers describe CMOS sensor arrays for detecting DNA hybridization
using electrochemical methods, microelectromechanical resonator methods for measuring mass and volume, paper fluidic lab-on-a-chip systems, a membrane microvalve system for controlled droplet generation, and Hall effect-based magnetic immunosensors.
In previously studies DNA hybridization
was used for identification of different phytoplasma associated with peanut sweet potato witches'-broom and other phytoplasma disease (Chen and Lin 1997; Ko and Lin 1994).
detection is a main issue in molecular biodiagnostics , determination of genetic diversity , food analysis [10,11], criminal investigation in forensics and immigration , and environmental monitoring .
Like IHC, fluorescence in situ hybridization (FISH)-based detection of the ERBB2 [v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian); also known as HER2] gene copy number is a morphologydriven slide-based assay that features DNA hybridization
with fluorescently labeled probes (9).
experiments were performed by the method of Southern, as described by Sambrook et al.