DNA hybridization

DNA hy·brid·i·za·tion

a technique used to determine the relatedness of microorganisms by the speed and efficiency of the reassociation of single-stranded DNA to form double-stranded DNA when one of the strands originates from one organism and the other strand from another organism; occurs when the base sequences are complementary or nearly so.

DNA hybridization

Molecular medicine A technique for determining the presence of a target DNA in a sample of tissue or cells. See HLA analysis, Paternity testing, RFLP analysis.

DNA hybridization

The use of radioactive known segments of DNA to determine the presence of complementary strands in a sample so as to determine identity, or to assess the degree of similarity between two individuals.
References in periodicals archive ?
A large number of systems based on DNA hybridization detection have been currently developed.
Man et al., "Real-time reliable determination of binding kinetics of DNA hybridization using a multi-channel graphene biosensor," Nature Communications, vol.
Zhao, "Kinetics and thermodynamics of DNA hybridization on gold nanoparticles," Nucleic Acids Research, vol.
When DNA hybridization is detected electrically, CNT nano-bioconjugates for DNA detection are deposited on electrode surfaces.
Another moderately rapid test is the Affirm VPIII Microbial Identification Test (Becton Dickinson; Sparks, MD), which relies on direct DNA hybridization. This test does not "amplify" the DNA present so it is not as sensitive as the newest types of diagnostic tests cleared by the FDA (see nucleic acid amplification tests [NAATs], below).
and Maeda, M., Rapid aggregation of gold nanoparticles induced by non-cross-linking DNA hybridization. Journal of the American Chemical Society, 2003; 125(27): 8102-8103.
The nine papers describe CMOS sensor arrays for detecting DNA hybridization using electrochemical methods, microelectromechanical resonator methods for measuring mass and volume, paper fluidic lab-on-a-chip systems, a membrane microvalve system for controlled droplet generation, and Hall effect-based magnetic immunosensors.
In previously studies DNA hybridization was used for identification of different phytoplasma associated with peanut sweet potato witches'-broom and other phytoplasma disease (Chen and Lin 1997; Ko and Lin 1994).
DNA hybridization detection is a main issue in molecular biodiagnostics [8], determination of genetic diversity [9], food analysis [10,11], criminal investigation in forensics and immigration [12], and environmental monitoring [8].
Like IHC, fluorescence in situ hybridization (FISH)-based detection of the ERBB2 [v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian); also known as HER2] gene copy number is a morphologydriven slide-based assay that features DNA hybridization with fluorescently labeled probes (9).
DNA hybridization experiments were performed by the method of Southern, as described by Sambrook et al.