DNA helicases


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DNA helicases

Enzymes that bring about the unwinding of DNA strands prior to replication.
References in periodicals archive ?
In this approach, template DNA is mixed with two template-specific primers which are of opposite orientation and flank the region of interest (just as in PCR), along with a DNA helicase, some SSBs, and often (depending on the specific helicase and polymerase), a few other "accessory proteins" which assist the helicase and DNA polymerase in optimum function.
It is caused by null mutations at WRN locus, which codes for a member of RecQ family of DNA helicases.6-9,11 The disease is associated with excessive synthesis of collagen type I and III which is dependent on elevated messenger RNA(mRNA) levels.6 The locus of Werner Syndrome has been found on the short arm of chromosome 8 in both Japanese and non Japanes.5,7,11 Fibroblasts isolated from WS patients exhibit genomic instability, increased sensitivity to specific DNA damaging agents, slow proliferation, lengthened S-phase, and accelerated replicative senescence.5,7,16
Other topics include mediation and modulation of membrane-cytoskeleton interactions, metal binding affinity and selectivity in metalloproteins, translocation and unwinding mechanisms of RNA and DNA helicases, and the structure of eukaryotic RNA polymerases.