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* UV-gel box to visualize DNA fragments in AGE and DGGE gels (e.g., Fisher Scientific)
Los perfiles de bandas que se obtuvieron a partir de las corridas electroforeticas (DGGE) fueron convertidos en una matriz binaria de acuerdo a la presencia o ausencia de bandas.
For the DGGE technique a polymerase chain reaction (PCR) was performed initially with universal primers set for bacteria based on 16S rRNA gene, U968f-GC1 and L1401.
Different colonization and residence time of Listonella anguilarum and Vibrio splendidus in the rotifer Brachionus plicatilis determined by real-real time PCR and DGGE. Aquaculture 302 (2010) 26-35.
DGGE genetic fingerprints were performed by the Bio-Rad DCodeTM Universal Mutation Detection System (Bio-Rad Laboratories, Hercules, CA, USA).
Abbreviations are defined as follows: qPCR, quantitative PCR; DGGE, denaturing gradient gel electrophoresis; ITS, intergenic transcribed spacer analysis; TRFLP, terminal restriction fragment length polymorphism analysis; PLFA, phospholipid fatty acid analysis; TSFAME, total soil fatty acid methyl ester analysis (Fig.
Most commonly, a prescreening method such as denaturing high-performance liquid chromatography (dHPLC) [3] or denaturing gradient gel electrophoresis (DGGE) is used, followed by sequencing of aberrant fragments.
fingerprinting technique, called denaturing gradient gel electrophoresis (DGGE), which is used to evaluate microbial ecology.
The present study is designed to develop a simple methodology to verify presence or absence of heteroduplexes in PCR samples which in conjunction with 'reconditioned' PCR can be used as an effective control to indicate presence and positions of HDs during DGGE analysis.
Slurry samples were periodically taken from the microcosms for analysis of community rDNA using denaturing gradient gel electrophoresis (DGGE).
Denaturing gradient gel electrophoresis (DGGE) was used to characterize the alleles present in the amplified samples.
Polymerase chain reaction products can be detected by agarose and polyacrylamide gel electrophoresis, single-strand conformational polymorphism, heteroduplex analysis, denaturing gradient gel electrophoresis (DGGE), temperature gradient gel electrophoresis (TGGE), and capillary gel electrophoresis.[5-12]