and "shell vial" cell culture were applied in all samples simultaneously.
Anti-rabies antibodies are labeled with this fluorochrome in the DFA test
A positive DFA test
led to immediate treatment with cidofovir, to which the patient responded and then recovered without needing a liver transplant.
On September 3, the available information included the bear's clinical presentation of acute death atypical for but consistent with rabies; the initial positive DFA test
and the positive PCR test at ISU VDL; the negative tests conducted by CDC on the bear's brain and spinal cord; the negative DFA test
conducted by UHL on the bear's brain; a documented case of a rabid bear with a DFA-negative test on brain tissue ; the paucity of literature on rabies and rabies testing in bears, and follow-up of humans after exposure to animals with negative laboratory results; and the lack of a reasonable alternative explanation for the bear's neurologic illness and death.
The marked insensitivity of the DFA test
, however, precluded the use of this methodology for analysis of discordant results.
The diagnosis was confirmed at CDC using the DFA test
and reverse transcriptase polymerase chain reaction (RT-PCR) on brain tissue samples.
A DFA test
of this isolate was positive, demonstrating the presence of F1 antigen, which is unique to Y.
The study showed a dramatic decrease in the use of IV antibiotics at a children's hospital after introduction of the DFA test
Additional brain tissue sent to CDC for confirmation tested positive on February 28 for rabies antigen by the DFA test
samples tested Preservation dRIT CDC DFA test
CDC Group A.
Stool specimens from two of the persons whose illnesses met the case definition were obtained by MDH 7 days after resolution of their symptoms; one sample was positive for oocysts and Cryptosporidium sporozoites on acid-fast staining, but the DFA test
Both a DFA test
and culture performed on nasopharyngeal secretions obtained on June 8 confirmed the persistence of pertussis organisms, and intravenous erythromycin therapy (30 mg/kg/day) was initiated.