In this work, Immunocytochemistry, immunofluorescence and Western-blot were performed to detect the expression of neurexophilin 1 in epithelial cells of chicken utero-vaginal junction, chicken embryo fibroblasts (DF-1 cells) and Hela cells.
Chicken, Neurexophilin 1, DF-1 cells, Hela cells, Genital tract.
This study will detect the expression of neurexophilin 1 gene in the epithelial cells of the UVJ, DF-1 cells and Hela cells, aims to look for convenient cells when studying the function of neurexophilin 1 gene in chicken genital tract.
The DF-1 cells and Hela cells were cultured in DMEM medium, supplemented with 10% fetal bovine serum at 37AdegC in a 5% CO2 incubator.
Firstly, we conducted immunohistochemistry to examine the subcellular localizations of NXPH1 in chicken genital tract epithelial cells and DF-1 cells.
Then immunofluorescence was used to detect the distribution of NXPH1 in DF-1 and Hela cells.
The SHC can burn a full range of liquid fuels, including DF-2, DF-1
, DF-A, JP-5 and JP-8, which provides for maximum flexibility and control.
Amid crucial talks here that could see German media kingpin Kirch Group take control of the local pay TV market, signs are that Kirch is gearing up to announce it will halve the purchase price for the d-Box decoder necessary to receive its digital platform DF-1 and pay TV web Premiere.
On sale at between DM900-DM1000 ($502-$558), the d-Box will be available in conjunction with a subscription to DF-1 for $279 starting in May.
In other news, Kirch has managed to merge his digital TV platform, DF-1
, with Premiere, the pay-TV service Kirch jointly owns with CLT-UFA.