Regulation of MicroRNA 183 by Cyclooxygenase 2 in Liver is DEAD-Box Helicase p68 (DDX5
) Dependent: role in insulin signaling.
Shen et al., "DDX5
is a positive regulator of oncogenic NOTCH1 signaling in T cell acute lymphoblastic leukemia," Oncogene, vol.
2007; Schroder 2010).p68 (DDX5) is a prototypic member of the DEAD box family (Ford et al.
The sequence alignment between clone and known DDX5 sequence was performed by using CLUSTAL W software.
BLAST (Basic Local Alignment Search Tool) has shown that this partial p68 gene sequence is 99% similar with Pan troglodytes DDX5 mRNA (AK306198.1) 94% similarity with DEAD box polypeptide 5 (DDX5) mRNA (NM_001191395.1) of Bos taurus and DDX5 mRNA (NM_001007613.1) of Rattus norvegicus was 93% similar.
To study the genetic evolution of the human DDX5 a phylogenetic tree (Fig.
It is suggested that DDX5 of Human sapiens and of Sus scrofa and Pan troglodytes have originated from same ancestors.
Some transcription factors, such as p53, receptor-regulated SMADs (R-Smads), and estrogen receptor a (ER[alpha]), can also participate in the rapid regulation of miRNA expression in response to extracellular stimuli by interacting with the DEAD-box RNA helicases p68 (DDX5
) and/or p72 (DDX17).
Primers sequences for reference genes: B2M, GAPDH, HMBS, ACTB, DDX5
and gene of interest COMMD1 were selected from http://primerdepot.nci.nih.gov/ database .
These methods (23,45) have provided evidence that EGCG binds to several intracellular proteins such as vimentin and the ATP-dependent RNA helicase DDX5
, indicating that EGCG can enter into the cell.
The microprocessor complex also contains a variety of cofactors including the DEAD box (amino acid sequence D-E-A-D asp-glu-ala-asp) RNA helicases p68 (DDX5
) and p72 (DDX17), as well as heterogeneous nuclear ribonucleoproteins (hnRNPs) [11,12].
Human tumor cells (at left, each line represents one cell) that withstood a dose of the anticancer drug cam ptothecin made more of the DDX5
enzyme (as measured by fluorescence level), while enzyme levels dropped in the cells eventually killed by the treatment.