DDX4


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DDX4

A gene on chromosome 5p15.2-p13.1 that encodes a protein which contains a conserved Asp-Glu-Ala-Asp (DEAD) motif; it is expressed only in the ovaries and testes, and in migratory primordial germ cells near the gonadal ridge in both sexes. It may play a role in sperm motility.
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DDX4 was detected in the cytoplasm of spermatocyte and chromatoid body of round spermatid (Figure 2A).
DDX4 plays a role for RNA substrate exchange between pi-bodies and piP-bodies as predicted by the ping-pong mechanism (Reynolds et al.
TDRD12 was not co-localized with either DDX4 or TDRD1 during spermatogenesis.
2] in PBS for 15 mins to block endogenous peroxidase and then incubated with anti Oct4, Nanog, SSEA4, DDX4 and C-kit antibody overnight at 4 [degrees]C.
Real time PCR analysis: Expression of germ cell specific genes such as OCT4, STELLA, SCP3 and DDX4 was detected in treated and control hUCMSC groups by Real time PCR analysis.
The transcription factors such as DDX4 were expressed in post-migratory primordial germ cells until the premeiotic stage of germ cells.
The addition of exogenous factors such as BMP-4, BMP-7 and BMP-8 to stem cell cultures increases the number of germ cells expressing DDX4, but does not necessarily induce their progress in meiosis (25).
The academics, led by Dr Jonathan Tilly of Massachusetts General Hospital, managed to identify and extract human stem cells that can go on to become immature eggs, because all carry a unique protein called DDX4.
To characterize the concentration, integrity, and stability of cfsRNA, we selected the transcripts of 2 genes: ACTB (actin, beta) [5] and DDX4 [DEAD (AspGlu-Ala-Asp) box polypeptide 4], a germ cell-specific gene.
We used full-length PCR amplicons of human ACTB and DDX4 transcripts as calibrators to quantify their different regions.
MEASUREMENT OF DIFFERENT PARTS OF ACTB AND DDX4 mRNAs
Early in human development, Smith explains, cells that eventually become part of the male or female reproductive system express two proteins, DDX4 and POU5fl.