However, due to the high cost many clinical microbiology laboratories use alternative methods such as the double-disc synergy test (
DDST) and the combined disc (CD)/disc potentiation test (DPT).
In regard to developmental assessment, 741 out of the 1000 subjects (74.1%) had normal, 140 (14%) had risky, and 119 (11.9%) had abnormal findings on the
DDST results.
The DDDT test was used to confirm that strains were positive by
DDST. Standardized inoculum conforming to 0.5 McFarland standards of suspected ESBL-producing isolates were inoculated as previously described.
In addition, they deployed a
DDST to Port Arthur, Texas, and several personnel to support sister battalion operations in San Diego and Philadelphia.
with imipenem-EDTA
DDST or alternative
DDSTs, such as those using 2-mercaptopropionic acid, which appears to be more sensitive for detecting MBLs among Acinetobacter spp.
ESBL screening using
DDST and CDDT showed that 110 (73.3%) isolates were confirmed as ESBL belonging to female patients and age groups of 16-35 (56.7%) and 36-50 (16.7%) years old.
Among the CP strains, MBL was detected in 134/138 (97.1%) isolates both by CDT and
DDST including 90 (67.16%) Klebsiella pneumoniae and 44 (32.84%) E.coli.
Comparison of
DDST and CLSI confirmatory test showed that the
DDST detected 145 (67.8%) isolates while 213 (99.5%) ESBL K.
* The 839th Battalion Headquarters and
DDST supported exercise Natural Fire 10 by conducting cargo download and upload operations in Mombasa, Kenya and coordinating onward movement to training areas in Kampala, Uganda.
Phenotypic identification for ESBL production was confirmed by double disk synergy test (
DDST) and phenotypic confirmatory double disk test (PCDDT) according to CLSI guidelines.
However, due to high cost many laboratories use alternative methods as the
DDST and DPT.