When there were fewer than 50 cells discernible by DAPI stain
, then all cells were examined, and the MYB FISH signals were recorded.
Another 10 ul of haemolymph suspension was placed on a glass slide and stained with DAPI stain (4, 6-diamidino-2-phenylindole) (Kapuscinski, 1995) to observe morphology of the nucleus and to confirm appearance (shape and structure) of nucleus of haemo-lymphocytes by using fluorescent microscopy.
2); the nucleus contained very high density of granules with blue fluorescence color, but DAPI stain could not stain cytoplasm.
Tenders are invited for Supply of 8 items BCR-ABL (dual colour, dual fusion) 20 test per kit, PML-RARA (dual colour, dual fusion) 20 test per kit, AML-ETO (dual colour, dual fusion) 20 test per kit, inv (16) (break apart) 20 test per kit, XY (centromere specific) 20 test per kit, Hybridization Buffer, 20 x SSC, DAPI Stain
is the most widely used fluorochrome to stain nuclear DNA in fluorescence microscopy.
I'm impressed by the compactness and conciseness of the DAPI stain
and the exclusion of your probe.
Representative images shown were superimposed images of TUNEL and DAPI stains
The DAPI stains
only the nuclei, allowing enumeration of sperm with relative ease (see Sakaluk 1984 for details of a similar method using Hoechst 33258 stain).