In order to know if the fluorescent pattern was not an artifact, we performed a DAPI stain
to see the nuclei of parasites into the PRBC (Fig.
Another 10 ul of haemolymph suspension was placed on a glass slide and stained with DAPI stain
(4, 6-diamidino-2-phenylindole) (Kapuscinski, 1995) to observe morphology of the nucleus and to confirm appearance (shape and structure) of nucleus of haemo-lymphocytes by using fluorescent microscopy.
When there were fewer than 50 cells discernible by DAPI stain
, then all cells were examined, and the MYB FISH signals were recorded.
is the most widely used fluorochrome to stain nuclear DNA in fluorescence microscopy.
I'm impressed by the compactness and conciseness of the DAPI stain
and the exclusion of your probe.
B, An isolated abnormal cell with multiple copies of loci 3q26 (red signal) and 5p15 (green signal) (DAPI stain
, original magnification x630).
Representative images shown were superimposed images of TUNEL and DAPI stains
Treatment of H37Rv-infected neutrophils with GSH-enhancing agents did not result in any loss in the viability of neutrophils as evident from the quantification of DAPI stained
cells (Figure 1(b)).
Lasers used were the Coherent UV with excitation lines of 351 and 361 nm to visualize DAPI stained
nuclei and a Uniphase argon laser line at 100% in the Acousto-Optical tuneable filter for the visualization of the Al-lumogallion complex.
Panel A: DAPI stained
nuclei in intact Dictyostelium cells.
Caption: Figure 5: Scanning Microscopy through DAPI stained
cells: the MCF-7 cells were stained with DAPI after 48 hrs of treatments.