Analysis was performed on a Cytomics
FC 500 using CXP software (Beckman Coulter).
Spectral imaging perspective on cytomics
. Cytometry A.
Standardization of platelet derived microparticle counting using calibrated beads and a Cytomics
FC500 routine flow cytometer: A first step towards multicenter studies?
Cells were harvested and stained with fluorescein isothiocyanate (FITC)-conjugated annexin V and PI according to the manufacturer's protocols using the annexin V-FITC/PI Kit (Cwbiotech, Beijing, China) and passed through a Cytomics
FC500 flow cytometry system.
(1) Laboratory of Biotechnology and Cytomics
, University of Aveiro, Campo Santiago, 3810-193 Aveiro, Portugal
We also used Micro PET/CT (Inveon, Siemens, Germany); [gamma]-counter (TY6017, Wizard, PerkinElmer, USA); flow cytometry (Cytomics
FC 500, Beckman, USA); Olympic BX51 fluorescence microscope (Olympus America Inc., Center Valley, PA).
Cells were analyzed using the Beckman Cytomics
FC500 Flow Cytometry Analyzer (Beckman Coulter, USA).
Fixed cell suspensions were subjected to multicolor flow cytometry (Cytomics
FC-500; Beckman Coulter).
After 15min incubation at room temperature in the dark, 400 [micro]L of 1x annexin binding buffer was added and cells were acquired by a flow cytometer (Cytomics
FC 500; Beckman Coulter, Fullerton, CA, USA).
Cytogenetic analysis was performed using 5 Colour Cytomics
FC500 flow cytometer and the banding method was GTG banding.
Impact of the New Beckman Coulter Cytomics
FC 500 5-Color Flow Cytometer on a Regional Flow Cytometry Clinical Laboratory Service.