The compounds isolated from Phoma exigua were named phomin and dehydrophomin and those from Helminthosporium dematioideum were called cytochalasin A and B, dehydrophomin being identical to cytochalasin A and phomin to cytochalasin B
. Soon afterwards, the ICI group isolated cytochalasins C and D from Metarrhizium spp.
In Crassostrea gigas, inhibition of PB1 with cytochalasin B resulted in a number of possible chromosome segregation patterns, including united bipolar, tripolar, and separated bipolar (3).
Genetic consequences of blocking polar body 1 with cytochalasin B in fertilized eggs of the Pacific oyster, Crassostrea gigas: I.
Morphogenesis of maternal and paternal genomes in fertilized oyster eggs (Crassostrea gigas): effects of cytochalasin B at different periods during meiotic maturation.
and Syracuse (N.Y.) University researchers have successfully packaged Cytochalasin B
-- a potential cancer drug -- in liposomes so that it is released gradually and its toxocity to healthy tissue is reduced.
To confirm whether the radially aligned birefringent fibers are actin-based, we examined the effect of externally applied cytochalasin B
. Cytochalasin B
is well known to prevent polymerization of actin filaments in growth cones of the Aplysia bag cell neuron (1).
: effects on membrane ruffling, growth cone and microspike activity, and microfilament structure not due to altered glucose transport.
Because cytochalasin B was dissolved in dimethylsulfoxide (DMSO) before dilution in ASW, the assay was also carried out in 1% DMSO-ASW as a control.
Colchicine, cytochalasin B, and phalloidin-FITC were from Sigma Chemical Co., St.
Cytochalasin B also inhibited the rounding, with 1 [[micro]gram]/ml of cytochalasin B being enough for complete inhibition.
Ethylenediaminetetraacetic acid (EDTA), caffeine, cytochalasin B and nor-ethyl-maleimide (NEM) were tested at several concentrations stated in the results.
Cytochalasin B (5 [[micro]gram]/ml)is the only chemical in the third category: it effectively inhibited hemocyte aggregation, but poorly blocked hemocyte adhesion.