Rotenone (0.1 [micro]M) showed 75% inhibition of estrogen activity in VM7Luc4E2 human breast carcinoma cells after 24 h, and reduced mitochondrial oxygen consumption in osteosarcoma cybrids
by 81% after 10 min (Figure 4A).
Relative mtDNA copy number for each cybrid
was obtained by comparing to the average Ct value of mtDNA from 21 cybrids
(2 [average Ct mtDNA-[Ct mtDNA-Ct 18S DNA]]).
(2005) Severe impairment of complex I-driven adenosine triphosphate synthesis in leber hereditary optic neuropathy cybrids
. Arch Neurol 62: 730-736.
Because transmitochondrial cybrid
cells are often used to verify the pathogenicity of mitochondrial DNA (mtDNA) mutations, we included both control and complex I-deficient (ND1 mutation) cybrid
The recent debate over human-animal hybrids, or cybrids
, has positioned the right-to-life stance on the fringes of public debate.
Production and characterization of intergeneric diploid cybrids
derived from symmetric fusion between Microcitrus papuana Swingle and sour orange (Citrus aurantium).
(54) He describes a method for removing mitochondrial DNA and replacing it with the mitochondria from platelets, creating a combination of nucleated and nonnucleated cells called cybrids
. This method has been used to evaluate the role of mitochondria DNA genes on the pathogenesis of neurodegenerative disorders.
These cells, called cybrids
, make it possible to study the effect of distinct mtDNA mutations on ATP production, protein synthesis, and other important mitochondrial processes within individual cells.
were not able to create long-term cybrids
from the mouse-human fusion, although they were able to achieve it from mouse-mouse fusions.
Wong, "Mitochondrial dysfunction in human breast cancer cells and their transmitochondrial cybrids
," Biochimica et Biophysica Acta (BBA)--Bioenergetics, vol.
To homogenize nuclear and environmental factors, we built transmitochondrial cell lines (cytoplasmic hybrids or cybrids
) with osteosarcoma 143B or adenocarcinoma A549 [rho.sup.0] nuclear backgrounds using patient and control platelets (Chomyn et al.
The reduction of complex I activity was also detected in cybrids
derived from PD patients, pointing to a mitochondrial DNA (mtDNA) impairment.