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MtCIDAS was measured in permeabilized compound and rotenone-treated (22 h + 2 h) cybrid cells containing LHON mutation (11,778) using a bioluminescence method.
This entirely mutation-dependent reduction in energy supply is supported by studies using cybrids carrying LHON mutations as well as peripheral lymphocytes from patients and mutation carriers [13].
Production and characterization of intergeneric diploid cybrids derived from symmetric fusion between Microcitrus papuana Swingle and sour orange (Citrus aurantium).
However, a TBTC concentration [greater than or equal to] 60 nM was required to affect the MIMP of the Awt cybrid (Figure 3).
A decline in complex I-sustained cell respiration and ATP production has been reported in assays performed on isolated mitochondria derived from muscle, Epstein-Barr Virus- (EBV-) transformed leukocytes, peripheral blood mononuclear cells (PBMCs), and cybrids [11-15].
To address cyber threats in CSDP, Estonia is organising in cooperation with the European Defence Agency a strategic table-top cyber-exercise EU CYBRID 2017 for EU Ministers of Defence during their informal meeting in Tallinn on 7 September 2017.
We have been using cybrid studies to evaluate inheritance patterns in RC disorders (10), which requires isolation and evaluation of many clones (>20).
The robotic combat simulation game, which challenges players to conquer Cybrid forces to save the Earth, features an all-new RAZOR aircraft and mighty new HERCs in stunning SVGA combat fields, so now you can fly and fight as well as battle on the ground.
The clonal cybrid cells between the cells lacking mitochondrial DNA and fibroblasts from a patient with the A3243G mutation showed poor respiration, an increased ratio of lactate to pyruvate, and marked defects in the mitochondrial morphology and respiratory chain complex I and IV activities (19).
The origin of the complex I defect in PD has been investigated through cybrid technology (12).