In lens, transparency and light refractive properties are maintained by water soluble proteins known as crystallins
Furthermore, the study provides distribution patterns of ubiquitous and taxon specific crystallins
from Uromastyx hardwickii that is likely to be useful in understanding evolutionary lineage of this organism.
One of these, 'compound 29,' was found to prevent crystallins
from forming clumps in laboratory tests.
are the major component of fiber cells, which form the eyes' lenses, and the unique properties of these cells make them particularly susceptible to damage, said Jason Gestwicki, PhD, associate professor of pharmaceutical chemistry at UCSF and co-senior author of a paper on the new research, most of which was undertaken in Gestwicki's laboratory at the U-M Life Sciences Institute.
In order for our lenses to function well, this permanent, finite reservoir of crystallins
must maintain both the transparency of fiber cells and their flexibility, as the eyes muscles constantly stretch and relax the lens to allow us to focus on objects at different distances.
Moreover higher molecular weight [beta]B1 crystallins
were also found in all the samples by Westeren blotting using [beta]B1 crystallin
monoclonal antibodies which shows that glutathione reacted with [beta]B1 crystallin
and formed higher molecular weight protein adducts.
6-8) Structural proteins in the lens called crystallins
, when cross-linked, result in cataracts.
Identification and quantification of leucine aminopeptidase in aged normal and cataractous human lenses and ability of bovine lens LAP to cleave bovine crystallins
Quantification of chick lens [alfa] A - and [delta] crystallins
in experimentally induced ametropia.
In all cases the ELISA technique was carried out in an indirect configuration and using a secondary antibody-alkaline phosphatase conjugate since these were the basic conditions providing best sensitivity and consistency of results previously found in ELISAs designed to measure lens crystallins
in our laboratory (12).
Fernald (2006) gives an overview of eye evolution from a genetic standpoint, discussing the three protein families most associated with eyes: opsins, crystallins
, and regulatory genes that code for transcription factors.
Lens cells make a specific, predominant set of proteins called crystallins
," says bioorganic chemist Allen Taylor.