The ERC funding would allow my lab to pursue an ambitious interdisciplinary approach combining X-ray crystallography, cryo-electron microscopy
, biochemistry and cell biology.
May 19, 2015 /PRNewswire-iReach/ -- Structural biology is undergoing a seismic shift as single-particle cryo-electron microscopy
(cryo-EM) is increasingly becoming the technique of choice for scientists who want to study DNA, proteins and other biological molecules at atomic resolution.
At UMass Medical School, researchers are also enthusiastic about the possibilities of the new High Resolution Cryo-Electron Microscopy
facility the school will build with the life sciences grant.
The data generated from various methods such as X-ray crystallography, NMR spectroscopy, cryo-electron microscopy
, gel-electrophoresis alone or in combination are used to determine the structure of proteins.
Then, in separate studies, using first cryo-electron microscopy
and then X-ray crystallography, the researchers were able to reveal the structure of the Env trimer, how it assembles and how it interacts with broadly neutralizing antibodies that target HIV.
Zhou uses cryo-electron microscopy
(cryoEM) to produce atomically accurate three-dimensional models of biological samples such as viruses.
images reveal the details of a starfish-shaped structure (A, B) that covers an opening in the virus coat through which DNA might be expelled when the virus infects a host.
reveals human low density lipoprotein substructure.
Studies of the dengue structure were done using the techniques of cryo-electron microscopy
and three-dimensional image reconstruction.
High-field NMR spectroscopy will be combined with other progressive high-resolution methods of structural analysis such as single-crystal X-ray diffraction, high-end cryo-electron microscopy
and tomography, atomic force understanding of the vital cellular processes.
We aim to characterize the structural and mechanistic features of ranavirusassembly, maturation and cell entry by combination of cryo-electron microscopy
and tomography with othercomplementary techniques.
April 15, 2015 /PRNewswire-iReach/ -- For many years the lack of sub 3 Ea (angstrom) high-resolution structures solved by single particle cryo-electron microscopy
(cryo-EM) created speculation that 3 Ea might be the fundamental resolution limit of the technique.