They performed cryo-electron microscopy
(cryo-EM) of the drug-virus complex to determine how the drug exerted its effect.
Researchers used single-particle cryo-electron microscopy
to visualise the full-length PDE6 enzyme.
Megabodies are novel antigen-binding chimeric proteins which are ideally suited for three-dimensional structural analysis of proteins via high-resolution cryo-electron microscopy
can significantly accelerate scientists' understanding of molecular structures and processes, leading to positive impacts in understanding disease and the discovery of new drugs," Brookhaven Lab Director Doon Gibbs said in a statement.
Over the past five decades, scientists have been able to determine shapes of proteins in labs using experimental techniques like cryo-electron microscopy
, nuclear magnetic resonance or X-ray crystallography, but each method depends on a lot of trial and error, which can take years and cost tens of thousands of dollars per structure.
or Cyro-EM is a technique used for determining high-resolution structure of biomolecules.
(Last month, three pioneers of cryo-electron microscopy
won the 2017 Nobel Prize in Chemistry.) Researchers have long known that the mirror in a scallop eye is made from a molecule called guanine.
2017 Jacques Dubochet, Joachim Frank and Richard Henderson "for developing cryo-electron microscopy
for the high-resolution structure determination of biomolecules in solution"
Reason: For the development of cryo-electron microscopy
for high resolution structure determination of biomolecules in solution.
Jacques Dubochet, Joachim Frank and Richard Henderson were awarded the prize for their work in developing cryo-electron microscopy
, or cryo-EM, a technique that fires beams of electrons at proteins that have been frozen in solution to deduce the biomolecules' structure.