The slides were placed in coplin jar
with 0.01 M Tris-EDTA buffer at pH of 9.0.
After rinsing the slides with a gentle stream of water, slides were dipped in coplin jar
containing 25% [H.sub.2] S[O.sub.4] for 2-4 min, and if necessary, decolorisation step was repeated for another 1-3 minutes.
The product is available in a 1-L bottle for Coplin jars
or staining dishes and a 4-L container with flow control spout for filling baths on the Little Dipper Processor and VP 2000 instruments.
After incubation, the slides containing the microarrays were washed twice in phosphate-buffered saline (PBS) containing 0.5 mL/L Tween 20 by soaking for 2 min in Coplin jars
. Patient serum (10 [micro]L) was added to individual microarrays and incubated for 30 min at 37[degrees]C.