Coombs test


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Coombs test

(kūmz),
a test for antibodies, the so-called anti-human globulin test using either the direct or indirect Coombs tests.
Synonym(s): antiglobulin test

Coombs test

(kūmz test)
A procedure for measuring antibodies, the so-called anti-human globulin test, using either the direct or indirect Coombs tests.

Coombs test

(Koomz)
[R. R. A. Coombs, Brit. immunologist, b. 1921]
A laboratory test for the presence of antibodies, usually blood type antibodies, in serum. The patient's serum is incubated with red blood cells (RBCs) with known antigenic markers; if antibodies to the antigen are present in the serum, they bind with the RBCs. When antihuman globulin is added, RBC clumping (agglutination) occurs. The test is used for crossmatching blood before transfusions to ensure that no antigen-antibody reactions will occur and to test for the presence of specific antibodies to RBCs.

Coombs,

Robin R.A., English veterinarian and immunologist, 1921–.
Coombs serum - serum from a rabbit or other animal previously immunized with purified human globulin to prepare antibodies directed against IgG and complement. Synonym(s): antihuman globulin
Coombs test - a test for antibodies, the so-called antihuman globulin test, using either the direct or indirect Coombs tests. Synonym(s): antiglobulin test
direct Coombs test - a test for detecting sensitized erythrocytes in erythroblastosis fetalis and in cases of acquired immune hemolytic anemia.
Gell and Coombs reaction - see under Gell
indirect Coombs test - a test routinely performed in cross-matching blood or in the investigation of transfusion reaction.
References in periodicals archive ?
In our case there was thrombocy to penia with positive Coombs test. Steroid such as prednisone is the initial treatment of choice for Evans syndrome.
After taking history and performing physical examination, blood tests were run for complete blood count, peripheral smear, reticulocyte count, coombs test, serum bilirubin, mother and baby's blood group.
Important distinguishing features that may assist in diagnosing TTP in this context include the presence of schistocytes on peripheral smear, a negative Coombs test, normal complement levels, and a low level of ADAMTS13 activity.
A positive Coombs test requires cautious interpretation when there are no other features of autoimmune hemolytic anemia.
She was diagnosed with as autoimmune hemolytic anemia (ATHA) based on a high reticulocyte count, positive direct Coombs test, hemoglobinuria, and low haptoglobin level, and received the conventional treatment of prednisone (2 mg/kg in divided doses) and blood transfusions.
We detected autoantibodies on the membrane of BM hemopoietic cells by bone marrow mononuclear-cell-(BMMNC-) Coombs test [4-6] or flow cytometric analysis [7].
Other possible hemolytic anemia or enzyme deficiencies causes were excluded (negative Coombs test, normal glucose-6-phosphate dehydrogenase enzyme level, normal hemoglobin electrophoresis and bone marrow aspiration).
Blood work revealed low haptoglobin (< 8 mg/dL), LDH was 778 U/L, retic count was 29.3%, total bilirubin was 2.4 mg/dL, direct Coombs test was positive (warm, polyspecific IgG), and so was the indirect Coombs test.
The patient was negative for hepatitis A, B, and C virus antibodies and antimitochondrial antibodies and had a negative direct Coombs test result.
Diagnosis of CAHA is based on a positive direct Coombs test in the presence of cold agglutinins.
(This would pertain providing the intended recipient has a negative atypical antibody screen and direct Coombs test and a negative transfusion reaction history.)
The pro-thrombin time was 11.8sec, and indirect and direct Coombs test was negative.