Coomassie brilliant blue R-250

Coo·mas·sie bril·liant blue R-250

(kū'ma-sī' bril'i-yant blū), [C.I. 42660]
A general protein stain used in electrophoresis because of its unusual sensitivity.
[originally, a proprietary name of Imperial Chemical; Coomassie (Kumasi), Ghana]
References in periodicals archive ?
25% Coomassie brilliant blue R-250 stain (Amresco, USA) for 4 hours and then suitably destained for best visibility of the protein bands with several changes of destaining solution and stored in 7% glacial acetic acid solution for photography.
All the other chemicals used, including agarose, methyl green, agar, Triton X-100, Coomassie Brilliant Blue R-250 and bromophenol blue were of analytical grades.
The purified protein samples were seen as two or three smeared bands alter Coomassie brilliant blue R-250 staining (Figure 3A).
1 % w/v Coomassie Brilliant Blue R-250 (Sigma) in methanol:water:ethanoic acid (5:5:1) and then destained with a solution of methanol:water:ethanoic acid (1:17:2).
The efficiency of transfer was determined using Coomassie brilliant blue R-250, and then the membranes were tested for reactivity with antibodies in the B.
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis was performed in a 6% slab gel under nonreducing conditions (12), and the proteins were stained with Coomassie Brilliant Blue R-250.
Protein staining was carried out with Coomassie Brilliant Blue R-250 or Silver Stain II Kit (Wako Pure Chemical).