Coomassie blue

Coomassie blue

(kū′mă-sē) [Kumasi, Ghana (proprietary name)]
Anazolene sodium, a stain used to demonstrate proteins, e.g., in protein electrophoretic gels.
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For determination of the protein profiles, the testicular proteins (80 [micro]g) were loaded and separated on 10 % sodium dodecyl sulfate (SDS) polyacrylamide gel followed by Coomassie blue staining (SDS-PAGE).
Electrophoris was run at a constant voltage of 200V with gel stained for 12 hours with Coomassie Blue Brilliant R-250 0.
Coomassie Blue staining of proteins separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).
Gels were stained with Coomassie blue and 20 [micro]L samples were loaded perlane.
The purity of the purified protein was assessed using electrophoresis on the 12% polyacrylamide gel and subsequent Coomassie blue staining.
We excised well resolved spots from coomassie blue stained 2 DE gel by using Ex-Quest spot cutter (Bio-Rad).
As protein loading controls, the SDS-PAGE gel was stained with Coomassie blue (Figure 7(b)) and the blot was probed for GAPDH (Figure 7(b)).
The gel was stained with Coomassie Blue: The gel was incubated in Coomassie blue R-250 (0.
BSA concentrations were obtained from the Bradford method relying on the binding of the dye Coomassie Blue G250 to protein [32], The absorbance at 595 nm was determined by a UV-vis spectrophotometer (UV-1601, Shimadzu, Japan).
After staining the membrane with coomassie blue, the spots were excised and the N-terminal sequence of the proteins determined using an automated Edman degradation method with a Perkin Elmer Applied Biosystems Model 494 Procise protein sequencer (Foster City, CA, USA).
Protein preparation was subjectd to electrophoresis in SDS- polyacrylamide gel (12 %) (Laemmli 1970) [30] and protein was visualised by Coomassie blue staining.