The electrophoresis was performed at 80 V, the gel was stained with a Coomassie Blue
R-250 solution (ethanol 50% (v/v), acetic acid 10% (v/v), and Coomassie Blue
R-250 0,1% (w/v)) during 2 hours, and then it was discolored with a solution composed by ethanol 50% (v/v) and acetic acid 10% (v/v) until the bands visualization.
The membranes were stained with Coomassie blue
solution (1% Coomassie blue
, 10% acetic acid, 50% methanol) for 1 min and washed in bleach solution (5% acetic acid, 40% methanol) for 2 min with stirring (for verification of the efficiency of the transfer procedure).
After that, separated proteins were stained with Coomassie Blue
or transferred onto nitrocellulose membrane.
Bradford assay: Total protein content of different food extracts was measured by using coomassie blue
dye by allowing the mixing of 30 ul of food extracts to 300 ul of the reagent and placed the mixture at room temperature for 15 minutes.
Morphological evaluation of protein adsorption using Coomassie blue
The purified recombinant protein was analyzed by 12% SDS-PAGE followed by staining with Coomassie Blue
Quality control of the protein extracts were performed by SDS-PAGE and subsequent staining using Coomassie Blue
, as previously described .
Thorp, "Properties and biological behaviour of coomassie blue
," Heart, vol.
The efficacy of PROse or RNase enzyme treatments was detected by gel electrophoresis or Coomassie blue
G250 staining, respectively.
Levine, "Quantitation of protein on gels and blots by infrared fluorescence of Coomassie blue
and Fast Green," Analytical Biochemistry, vol.
The separated protein bands were stained in the gel using Coomassie blue
(Thermo Fisher Scientific, Bonn, Germany).