Protein pattern was observed by staining a gel with coomassie
brilliant blue R-250.
Lower molecular weight products of hydrolysis were formed, but they escaped detection by the Coomassie
Brilliant Blue staining method.
To ascertain the efficiency of protein transfer, the gels were stained with Coomassie
Approximately 700 protein spots were detected on the coomassie
stained gels and most of them were appeared in the pH range between 5 to 9 and molecular weight between 20 to 100 kDa.
A sample from the fractions containing IF obtained after purification on protein G was subjected to SDSPAGE, and the protein bands were visualized by Coomassie
These experiments involved protein staining (silver or Coomassie
blue) and demonstrated that loss was not specific to any protein species (35).
Further these colonies were stained with Coomassie
Brilliant Blue stain and observed under bright filed microscope for the presence of crystalline inclusions.
Recombinant ApoA5 protein was analyzed by SDS-PAGE followed by Coomassie
Before being dried and exposed to autoradiography, the polyacrylamide gels were stained with Coomassie
Brilliant Blue to determine that equal amounts of fusion proteins were used in each reaction.
The proteins resolved by electrophoresis were stained with Coomassie
brilliant blue (Biorad) and were exposed to X-ray film (XOMAT AR, Eastman Kodak) for 24 h.
Staining of protein bands carried out by Coomassie
Brilliant Blue R250 and bands size were assessed by protein marker (Thermo scientific).