Both databases revealed identity in the range of 96-100% for consensus sequences
of three species (Ictalurus punctatus, I.
With the purpose of studying the amino acid (AA) substitutions along the NS5A protein, Uruguayan HCV AA sequences were aligned with NS5A world consensus sequences
(residues 23 to 354 relative to NS5A protein sequence).
After all the 16S rRNA sequences of 20 lactic acid bacteria were edited using the Bioedit software package, consensus sequences
obtained were blasted in GenBank of NCBI and only 10 samples showed significant similarity of 92-98% in the GenBank.
Wang et al., "A highly conserved G-rich consensus sequence
in hepatitis C virus core gene represents a new anti-hepatitis C target," Science Advances, vol.
The Weblogo server was used to generate a consensus HSE from the 18 predicted sites and the results exhibited that camel HSPA6 gene promoter was identical to the canonical HSE elements and the consensus sequence
deduced was nTTCnnGAAnnTTCn (Fig.
However, when the number of loci included in a particular sequencing run is increased, there is a need to optimize the uniformity of coverage because the genotyping and the consensus sequence
generated are only as good as the lowest point of coverage.
The Chr 1 consensus sequence
for each strain was constructed using the SAMtools consensus parameters.
HLA genotyping results and fully phased consensus sequences
were successfully generated for all 50 participants using the Omixon Twin algorithm (300 total alleles) and were found to be concordant with SBT/SSP genotyping results (2 field resolution, data not shown).
The consensus sequences
were subjected to BLAST analysis (Altschul et al.
Chromatograms obtained by sequencing were analyzed, and consensus sequences
were assembled using 4 sStaden package programs (STADEN et al., 2000).
According to the results of multiple alignments of all the obtained consensus sequences
, it was possible to detect differences between the species T.